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Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample...
Autores principales: | Hatakeyama, Keiichi, Wakabayashi-Nakao, Kanako, Aoki, Yutaka, Ogura, Shun-ichiro, Yamaguchi, Ken, Nakajima, Takashi, Sato, Taka-Aki, Mochizuki, Tohru, Hayashi, Isamu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352043/ https://www.ncbi.nlm.nih.gov/pubmed/22439923 http://dx.doi.org/10.1186/1477-5956-10-19 |
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