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Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample...

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Detalles Bibliográficos
Autores principales: Hatakeyama, Keiichi, Wakabayashi-Nakao, Kanako, Aoki, Yutaka, Ogura, Shun-ichiro, Yamaguchi, Ken, Nakajima, Takashi, Sato, Taka-Aki, Mochizuki, Tohru, Hayashi, Isamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352043/
https://www.ncbi.nlm.nih.gov/pubmed/22439923
http://dx.doi.org/10.1186/1477-5956-10-19

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