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Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development

Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by nat...

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Autores principales: Hooftman, Danny A P, Flavell, Andrew J, Jansen, Hans, den Nijs, Hans C M, Syed, Naeem H, Sørensen, Anker P, Orozco-ter Wengel, Pablo, van de Wiel, Clemens C M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352534/
https://www.ncbi.nlm.nih.gov/pubmed/25568012
http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x
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author Hooftman, Danny A P
Flavell, Andrew J
Jansen, Hans
den Nijs, Hans C M
Syed, Naeem H
Sørensen, Anker P
Orozco-ter Wengel, Pablo
van de Wiel, Clemens C M
author_facet Hooftman, Danny A P
Flavell, Andrew J
Jansen, Hans
den Nijs, Hans C M
Syed, Naeem H
Sørensen, Anker P
Orozco-ter Wengel, Pablo
van de Wiel, Clemens C M
author_sort Hooftman, Danny A P
collection PubMed
description Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by natural conditions creates an adaptive mosaic of alleles from both parental species. We investigated segregation patterns after hybridization between lettuce (Lactuca sativa) and its wild relative, L. serriola. Three generations of hybrids (S(1), BC(1), and BC(1S1)) were grown in habitats mimicking the wild parent's habitat. As control, we harvested S(1) seedlings grown under controlled conditions, providing very limited possibility for selection. We used 89 AFLP loci, as well as more recently developed dominant markers, 115 retrotransposon markers (SSAP), and 28 NBS loci linked to resistance genes. For many loci, allele frequencies were biased in plants exposed to natural field conditions, including over-representation of crop alleles for various loci. Furthermore, Linkage disequilibrium was locally changed, allegedly by selection caused by the natural field conditions, providing ample opportunity for genetic hitchhiking. Our study indicates that when developing genetically modified crops, a judicious selection of insertion sites, based on knowledge of selective (dis)advantages of the surrounding crop genome under field conditions, could diminish transgene persistence.
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spelling pubmed-33525342012-05-24 Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development Hooftman, Danny A P Flavell, Andrew J Jansen, Hans den Nijs, Hans C M Syed, Naeem H Sørensen, Anker P Orozco-ter Wengel, Pablo van de Wiel, Clemens C M Evol Appl Original Articles Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by natural conditions creates an adaptive mosaic of alleles from both parental species. We investigated segregation patterns after hybridization between lettuce (Lactuca sativa) and its wild relative, L. serriola. Three generations of hybrids (S(1), BC(1), and BC(1S1)) were grown in habitats mimicking the wild parent's habitat. As control, we harvested S(1) seedlings grown under controlled conditions, providing very limited possibility for selection. We used 89 AFLP loci, as well as more recently developed dominant markers, 115 retrotransposon markers (SSAP), and 28 NBS loci linked to resistance genes. For many loci, allele frequencies were biased in plants exposed to natural field conditions, including over-representation of crop alleles for various loci. Furthermore, Linkage disequilibrium was locally changed, allegedly by selection caused by the natural field conditions, providing ample opportunity for genetic hitchhiking. Our study indicates that when developing genetically modified crops, a judicious selection of insertion sites, based on knowledge of selective (dis)advantages of the surrounding crop genome under field conditions, could diminish transgene persistence. Blackwell Publishing Ltd 2011-09 2011-04-27 /pmc/articles/PMC3352534/ /pubmed/25568012 http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x Text en © 2011 Blackwell Publishing Ltd
spellingShingle Original Articles
Hooftman, Danny A P
Flavell, Andrew J
Jansen, Hans
den Nijs, Hans C M
Syed, Naeem H
Sørensen, Anker P
Orozco-ter Wengel, Pablo
van de Wiel, Clemens C M
Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title_full Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title_fullStr Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title_full_unstemmed Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title_short Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
title_sort locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for gm crop development
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352534/
https://www.ncbi.nlm.nih.gov/pubmed/25568012
http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x
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