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Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development
Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by nat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352534/ https://www.ncbi.nlm.nih.gov/pubmed/25568012 http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x |
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author | Hooftman, Danny A P Flavell, Andrew J Jansen, Hans den Nijs, Hans C M Syed, Naeem H Sørensen, Anker P Orozco-ter Wengel, Pablo van de Wiel, Clemens C M |
author_facet | Hooftman, Danny A P Flavell, Andrew J Jansen, Hans den Nijs, Hans C M Syed, Naeem H Sørensen, Anker P Orozco-ter Wengel, Pablo van de Wiel, Clemens C M |
author_sort | Hooftman, Danny A P |
collection | PubMed |
description | Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by natural conditions creates an adaptive mosaic of alleles from both parental species. We investigated segregation patterns after hybridization between lettuce (Lactuca sativa) and its wild relative, L. serriola. Three generations of hybrids (S(1), BC(1), and BC(1S1)) were grown in habitats mimicking the wild parent's habitat. As control, we harvested S(1) seedlings grown under controlled conditions, providing very limited possibility for selection. We used 89 AFLP loci, as well as more recently developed dominant markers, 115 retrotransposon markers (SSAP), and 28 NBS loci linked to resistance genes. For many loci, allele frequencies were biased in plants exposed to natural field conditions, including over-representation of crop alleles for various loci. Furthermore, Linkage disequilibrium was locally changed, allegedly by selection caused by the natural field conditions, providing ample opportunity for genetic hitchhiking. Our study indicates that when developing genetically modified crops, a judicious selection of insertion sites, based on knowledge of selective (dis)advantages of the surrounding crop genome under field conditions, could diminish transgene persistence. |
format | Online Article Text |
id | pubmed-3352534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-33525342012-05-24 Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development Hooftman, Danny A P Flavell, Andrew J Jansen, Hans den Nijs, Hans C M Syed, Naeem H Sørensen, Anker P Orozco-ter Wengel, Pablo van de Wiel, Clemens C M Evol Appl Original Articles Gene escape from crops has gained much attention in the last two decades, as transgenes introgressing into wild populations could affect the latter's ecological characteristics. However, different genes have different likelihoods of introgression. The mixture of selective forces provided by natural conditions creates an adaptive mosaic of alleles from both parental species. We investigated segregation patterns after hybridization between lettuce (Lactuca sativa) and its wild relative, L. serriola. Three generations of hybrids (S(1), BC(1), and BC(1S1)) were grown in habitats mimicking the wild parent's habitat. As control, we harvested S(1) seedlings grown under controlled conditions, providing very limited possibility for selection. We used 89 AFLP loci, as well as more recently developed dominant markers, 115 retrotransposon markers (SSAP), and 28 NBS loci linked to resistance genes. For many loci, allele frequencies were biased in plants exposed to natural field conditions, including over-representation of crop alleles for various loci. Furthermore, Linkage disequilibrium was locally changed, allegedly by selection caused by the natural field conditions, providing ample opportunity for genetic hitchhiking. Our study indicates that when developing genetically modified crops, a judicious selection of insertion sites, based on knowledge of selective (dis)advantages of the surrounding crop genome under field conditions, could diminish transgene persistence. Blackwell Publishing Ltd 2011-09 2011-04-27 /pmc/articles/PMC3352534/ /pubmed/25568012 http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x Text en © 2011 Blackwell Publishing Ltd |
spellingShingle | Original Articles Hooftman, Danny A P Flavell, Andrew J Jansen, Hans den Nijs, Hans C M Syed, Naeem H Sørensen, Anker P Orozco-ter Wengel, Pablo van de Wiel, Clemens C M Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title | Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title_full | Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title_fullStr | Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title_full_unstemmed | Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title_short | Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development |
title_sort | locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for gm crop development |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352534/ https://www.ncbi.nlm.nih.gov/pubmed/25568012 http://dx.doi.org/10.1111/j.1752-4571.2011.00188.x |
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