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Tissue reaction to sealing materials: different view at biocompatibility
The biodegradability of root canal sealers in areas other than the root canal system is crucial to the overall success rate of endodontic treatment. The aim of the present study was to investigate, the cell and tissue reaction to GuttaFlow and AHPlus, both in vitro and in vivo. For the in vitro expe...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352657/ https://www.ncbi.nlm.nih.gov/pubmed/21159573 http://dx.doi.org/10.1186/2047-783X-15-11-483 |
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author | Ghanaati, S Willershausen, I Barbeck, M Unger, RE Joergens, M Sader, RA Kirkpatrick, CJ Willershausen, B |
author_facet | Ghanaati, S Willershausen, I Barbeck, M Unger, RE Joergens, M Sader, RA Kirkpatrick, CJ Willershausen, B |
author_sort | Ghanaati, S |
collection | PubMed |
description | The biodegradability of root canal sealers in areas other than the root canal system is crucial to the overall success rate of endodontic treatment. The aim of the present study was to investigate, the cell and tissue reaction to GuttaFlow and AHPlus, both in vitro and in vivo. For the in vitro experiments the materials were incubated with Human Periodontal Ligament Fibroblasts and cell proliferation and cytotoxicity analyses were performed. Additional fluorescence-microscope stainings were carried out in order to visualize cell growth and morphology. For assessment of the tissue reaction to the materials a subcutaneous implantation model in Wistar rats was employed and the inflammatory response to the materials was visualized by means of general and specific histology after 6 weeks. Human gingival fibroblasts proliferation seemed to be dependent upon dental material and cultivation time. After an incubation period of 96 hrs AHPlus proved to be significantly (p < 0.002) more cytotoxic than GuttaFlow, as only a small number of fibroblasts survived on AHPlus. In vivo, GuttaFlow was surrounded by a fibrous capsule and no degradation took place, while AHPlus induced a well-vascularized granulation tissue in which the material was phagocyted by macrophages. The results of this study demonstrate that a potential cytotoxic effect of a sealing material may beneficial in order to have antibacterial properties and induce self degradation when accidentally extruded over the apical foramen. |
format | Online Article Text |
id | pubmed-3352657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-33526572012-05-16 Tissue reaction to sealing materials: different view at biocompatibility Ghanaati, S Willershausen, I Barbeck, M Unger, RE Joergens, M Sader, RA Kirkpatrick, CJ Willershausen, B Eur J Med Res Research The biodegradability of root canal sealers in areas other than the root canal system is crucial to the overall success rate of endodontic treatment. The aim of the present study was to investigate, the cell and tissue reaction to GuttaFlow and AHPlus, both in vitro and in vivo. For the in vitro experiments the materials were incubated with Human Periodontal Ligament Fibroblasts and cell proliferation and cytotoxicity analyses were performed. Additional fluorescence-microscope stainings were carried out in order to visualize cell growth and morphology. For assessment of the tissue reaction to the materials a subcutaneous implantation model in Wistar rats was employed and the inflammatory response to the materials was visualized by means of general and specific histology after 6 weeks. Human gingival fibroblasts proliferation seemed to be dependent upon dental material and cultivation time. After an incubation period of 96 hrs AHPlus proved to be significantly (p < 0.002) more cytotoxic than GuttaFlow, as only a small number of fibroblasts survived on AHPlus. In vivo, GuttaFlow was surrounded by a fibrous capsule and no degradation took place, while AHPlus induced a well-vascularized granulation tissue in which the material was phagocyted by macrophages. The results of this study demonstrate that a potential cytotoxic effect of a sealing material may beneficial in order to have antibacterial properties and induce self degradation when accidentally extruded over the apical foramen. BioMed Central 2010-11-25 /pmc/articles/PMC3352657/ /pubmed/21159573 http://dx.doi.org/10.1186/2047-783X-15-11-483 Text en Copyright ©2010 I. Holzapfel Publishers |
spellingShingle | Research Ghanaati, S Willershausen, I Barbeck, M Unger, RE Joergens, M Sader, RA Kirkpatrick, CJ Willershausen, B Tissue reaction to sealing materials: different view at biocompatibility |
title | Tissue reaction to sealing materials: different view at biocompatibility |
title_full | Tissue reaction to sealing materials: different view at biocompatibility |
title_fullStr | Tissue reaction to sealing materials: different view at biocompatibility |
title_full_unstemmed | Tissue reaction to sealing materials: different view at biocompatibility |
title_short | Tissue reaction to sealing materials: different view at biocompatibility |
title_sort | tissue reaction to sealing materials: different view at biocompatibility |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352657/ https://www.ncbi.nlm.nih.gov/pubmed/21159573 http://dx.doi.org/10.1186/2047-783X-15-11-483 |
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