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Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos

Silica nanoparticles can be efficiently employed as carriers for therapeutic drugs in vitro. Here, we use zebrafish embryos as a model organism to see whether mesoporous silica nanoparticles (MSNPs) can be incorporated to deliver compounds in vivo. We injected 35–40 nL (10 mg/mL) of custom-synthesiz...

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Autores principales: Sharif, Faiza, Porta, Fabiola, Meijer, Annemarie H, Kros, Alexander, Richardson, Michael K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352692/
https://www.ncbi.nlm.nih.gov/pubmed/22605936
http://dx.doi.org/10.2147/IJN.S26547
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author Sharif, Faiza
Porta, Fabiola
Meijer, Annemarie H
Kros, Alexander
Richardson, Michael K
author_facet Sharif, Faiza
Porta, Fabiola
Meijer, Annemarie H
Kros, Alexander
Richardson, Michael K
author_sort Sharif, Faiza
collection PubMed
description Silica nanoparticles can be efficiently employed as carriers for therapeutic drugs in vitro. Here, we use zebrafish embryos as a model organism to see whether mesoporous silica nanoparticles (MSNPs) can be incorporated to deliver compounds in vivo. We injected 35–40 nL (10 mg/mL) of custom-synthesized, fluorescently-tagged 200 nm MSNPs into the left flank, behind the yolk sac extension, of 2-day-old zebrafish embryos. We tracked the distribution and translocation of the MSNPs using confocal laser scanning microscopy. Some of the particles remained localized at the injection site, whereas others entered the bloodstream and were carried around the body. Embryo development and survival were not significantly affected by MSNP injection. Acridine orange staining revealed that MSNP injections did not induce significant cell death. We also studied cellular immune responses by means of lysC::DsRED2 transgenic embryos. MSNP-injected embryos showed infiltration of the injection site with neutrophils, similar to controls injected with buffer only. In the same embryos, counterstaining with L-plastin antibody for leukocytes revealed the same amount of cellular infiltration of the injection site in embryos injected with MSNPs or with buffer only. Next, we used MSNPs to deliver two recombinant cytokines (macrophage colony-stimulating factor and receptor for necrosis factor ligand) to zebrafish embryos. These proteins are known to activate cells involved in bone remodeling, and this can be detected with the marker tartrate-resistant acid phosphatase. Coinjection of these proteins loaded onto MSNPs produced a significant increase in the number of tartrate-resistant acid phosphatase-positive cells after 2–3 days of injection. Our results show that MSNPs can be used to deliver bioactive compounds into zebrafish larvae without producing higher mortality or gross evidence of teratogenicity.
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spelling pubmed-33526922012-05-17 Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos Sharif, Faiza Porta, Fabiola Meijer, Annemarie H Kros, Alexander Richardson, Michael K Int J Nanomedicine Original Research Silica nanoparticles can be efficiently employed as carriers for therapeutic drugs in vitro. Here, we use zebrafish embryos as a model organism to see whether mesoporous silica nanoparticles (MSNPs) can be incorporated to deliver compounds in vivo. We injected 35–40 nL (10 mg/mL) of custom-synthesized, fluorescently-tagged 200 nm MSNPs into the left flank, behind the yolk sac extension, of 2-day-old zebrafish embryos. We tracked the distribution and translocation of the MSNPs using confocal laser scanning microscopy. Some of the particles remained localized at the injection site, whereas others entered the bloodstream and were carried around the body. Embryo development and survival were not significantly affected by MSNP injection. Acridine orange staining revealed that MSNP injections did not induce significant cell death. We also studied cellular immune responses by means of lysC::DsRED2 transgenic embryos. MSNP-injected embryos showed infiltration of the injection site with neutrophils, similar to controls injected with buffer only. In the same embryos, counterstaining with L-plastin antibody for leukocytes revealed the same amount of cellular infiltration of the injection site in embryos injected with MSNPs or with buffer only. Next, we used MSNPs to deliver two recombinant cytokines (macrophage colony-stimulating factor and receptor for necrosis factor ligand) to zebrafish embryos. These proteins are known to activate cells involved in bone remodeling, and this can be detected with the marker tartrate-resistant acid phosphatase. Coinjection of these proteins loaded onto MSNPs produced a significant increase in the number of tartrate-resistant acid phosphatase-positive cells after 2–3 days of injection. Our results show that MSNPs can be used to deliver bioactive compounds into zebrafish larvae without producing higher mortality or gross evidence of teratogenicity. Dove Medical Press 2012 2012-04-11 /pmc/articles/PMC3352692/ /pubmed/22605936 http://dx.doi.org/10.2147/IJN.S26547 Text en © 2012 Sharif et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Sharif, Faiza
Porta, Fabiola
Meijer, Annemarie H
Kros, Alexander
Richardson, Michael K
Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title_full Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title_fullStr Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title_full_unstemmed Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title_short Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
title_sort mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352692/
https://www.ncbi.nlm.nih.gov/pubmed/22605936
http://dx.doi.org/10.2147/IJN.S26547
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