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Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease

Preclinical and clinical studies have shown that the application of CD105(+) mesenchymal stem cells (MSCs) is feasible and may lead to recovery after stroke. In addition, circulating microparticles are reportedly functional in various disease conditions. We tested the levels of circulating CD105(+)...

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Autores principales: Kim, Suk Jae, Moon, Gyeong Joon, Cho, Yeon Hee, Kang, Ho Young, Hyung, Na Kyum, Kim, Donghee, Lee, Ji Hyun, Nam, Ji Yoon, Bang, Oh Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352849/
https://www.ncbi.nlm.nih.gov/pubmed/22615882
http://dx.doi.org/10.1371/journal.pone.0037036
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author Kim, Suk Jae
Moon, Gyeong Joon
Cho, Yeon Hee
Kang, Ho Young
Hyung, Na Kyum
Kim, Donghee
Lee, Ji Hyun
Nam, Ji Yoon
Bang, Oh Young
author_facet Kim, Suk Jae
Moon, Gyeong Joon
Cho, Yeon Hee
Kang, Ho Young
Hyung, Na Kyum
Kim, Donghee
Lee, Ji Hyun
Nam, Ji Yoon
Bang, Oh Young
author_sort Kim, Suk Jae
collection PubMed
description Preclinical and clinical studies have shown that the application of CD105(+) mesenchymal stem cells (MSCs) is feasible and may lead to recovery after stroke. In addition, circulating microparticles are reportedly functional in various disease conditions. We tested the levels of circulating CD105(+) microparticles in patients with acute ischemic stroke. The expression of CD105 (a surface marker of MSCs) and CXCR4 (a CXC chemokine receptor for MSC homing) on circulating microparticles was evaluated by flow cytometry of samples from 111 patients and 50 healthy subjects. The percentage of apoptotic CD105 microparticles was determined based on annexin V (AV) expression. The relationship between serum levels of CD105(+)/AV(−) microparticles, stromal cells derived factor-1α (SDF-1α), and the extensiveness of cerebral infarcts was also evaluated. CD105(+)/AV(−) microparticles were higher in stroke patients than control subjects. Correlation analysis showed that the levels of CD105(+)/AV(−) microparticles increased as the baseline stroke severity increased. Multivariate testing showed that the initial severity of stroke was independently associated with circulating CD105(+)/AV(−) microparticles (OR, 1.103 for 1 point increase in the NIHSS score on admission; 95% CI, 1.032–1.178) after adjusting for other variables. The levels of CD105(+)/CXCR4(+)/AV(−) microparticles were also increased in patients with severe disability (r = 0.192, p = 0.046 for NIHSS score on admission), but were decreased with time after stroke onset (r = −0.204, p = 0.036). Risk factor profiles were not associated with the levels of circulating microparticles or SDF-1α. In conclusion, our data showed that stroke triggers the mobilization of MSC-derived microparticles, especially in patients with extensive ischemic stroke.
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spelling pubmed-33528492012-05-21 Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease Kim, Suk Jae Moon, Gyeong Joon Cho, Yeon Hee Kang, Ho Young Hyung, Na Kyum Kim, Donghee Lee, Ji Hyun Nam, Ji Yoon Bang, Oh Young PLoS One Research Article Preclinical and clinical studies have shown that the application of CD105(+) mesenchymal stem cells (MSCs) is feasible and may lead to recovery after stroke. In addition, circulating microparticles are reportedly functional in various disease conditions. We tested the levels of circulating CD105(+) microparticles in patients with acute ischemic stroke. The expression of CD105 (a surface marker of MSCs) and CXCR4 (a CXC chemokine receptor for MSC homing) on circulating microparticles was evaluated by flow cytometry of samples from 111 patients and 50 healthy subjects. The percentage of apoptotic CD105 microparticles was determined based on annexin V (AV) expression. The relationship between serum levels of CD105(+)/AV(−) microparticles, stromal cells derived factor-1α (SDF-1α), and the extensiveness of cerebral infarcts was also evaluated. CD105(+)/AV(−) microparticles were higher in stroke patients than control subjects. Correlation analysis showed that the levels of CD105(+)/AV(−) microparticles increased as the baseline stroke severity increased. Multivariate testing showed that the initial severity of stroke was independently associated with circulating CD105(+)/AV(−) microparticles (OR, 1.103 for 1 point increase in the NIHSS score on admission; 95% CI, 1.032–1.178) after adjusting for other variables. The levels of CD105(+)/CXCR4(+)/AV(−) microparticles were also increased in patients with severe disability (r = 0.192, p = 0.046 for NIHSS score on admission), but were decreased with time after stroke onset (r = −0.204, p = 0.036). Risk factor profiles were not associated with the levels of circulating microparticles or SDF-1α. In conclusion, our data showed that stroke triggers the mobilization of MSC-derived microparticles, especially in patients with extensive ischemic stroke. Public Library of Science 2012-05-15 /pmc/articles/PMC3352849/ /pubmed/22615882 http://dx.doi.org/10.1371/journal.pone.0037036 Text en Kim et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kim, Suk Jae
Moon, Gyeong Joon
Cho, Yeon Hee
Kang, Ho Young
Hyung, Na Kyum
Kim, Donghee
Lee, Ji Hyun
Nam, Ji Yoon
Bang, Oh Young
Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title_full Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title_fullStr Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title_full_unstemmed Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title_short Circulating Mesenchymal Stem Cells Microparticles in Patients with Cerebrovascular Disease
title_sort circulating mesenchymal stem cells microparticles in patients with cerebrovascular disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352849/
https://www.ncbi.nlm.nih.gov/pubmed/22615882
http://dx.doi.org/10.1371/journal.pone.0037036
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