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Monocytes Do Not Transdifferentiate into Proper Osteoblasts
Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to tw...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353470/ https://www.ncbi.nlm.nih.gov/pubmed/22623892 http://dx.doi.org/10.1100/2012/384936 |
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author | Schmitt, Andreas Ehnert, Sabrina Schyschka, Lilianna Buschner, Peter Kühnl, Andreas Döbele, Stefan Siebenlist, Sebastian Lucke, Martin Stöckle, Ulrich Nussler, Andreas K. |
author_facet | Schmitt, Andreas Ehnert, Sabrina Schyschka, Lilianna Buschner, Peter Kühnl, Andreas Döbele, Stefan Siebenlist, Sebastian Lucke, Martin Stöckle, Ulrich Nussler, Andreas K. |
author_sort | Schmitt, Andreas |
collection | PubMed |
description | Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to two previously published treatments to obtain unique, multipotent cell fractions, named programmable cells of monocytic origin (PCMOs) and monocyte-derived mesenchymal progenitor cells (MOMPs). Subsequently, MOMPs and PCMOs were treated with osteogenic differentiation medium (including either vitamin D or dexamethasone) for 14 days. Regarding a variety of surface markers, no differences between MOMPs, PCMOs, and primary monocytes could be detected. The treatment with osteogenic medium neither resulted in loss of hematopoietic markers nor in adoption of mesenchymal phenotype in all cell types. No significant effect was observed regarding the expression of osteogenic transcription factors, bone-related genes, or production of mineralized matrix. Osteogenic medium resulted in activation of monocytes and appearance of osteoclasts. In conclusion, none of the investigated monocyte cell types showed any transdifferentiation characteristics under the tested circumstances. Based on our data, we rather see an activation and maturation of monocytes towards macrophages and osteoclasts. |
format | Online Article Text |
id | pubmed-3353470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-33534702012-05-23 Monocytes Do Not Transdifferentiate into Proper Osteoblasts Schmitt, Andreas Ehnert, Sabrina Schyschka, Lilianna Buschner, Peter Kühnl, Andreas Döbele, Stefan Siebenlist, Sebastian Lucke, Martin Stöckle, Ulrich Nussler, Andreas K. ScientificWorldJournal Research Article Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to two previously published treatments to obtain unique, multipotent cell fractions, named programmable cells of monocytic origin (PCMOs) and monocyte-derived mesenchymal progenitor cells (MOMPs). Subsequently, MOMPs and PCMOs were treated with osteogenic differentiation medium (including either vitamin D or dexamethasone) for 14 days. Regarding a variety of surface markers, no differences between MOMPs, PCMOs, and primary monocytes could be detected. The treatment with osteogenic medium neither resulted in loss of hematopoietic markers nor in adoption of mesenchymal phenotype in all cell types. No significant effect was observed regarding the expression of osteogenic transcription factors, bone-related genes, or production of mineralized matrix. Osteogenic medium resulted in activation of monocytes and appearance of osteoclasts. In conclusion, none of the investigated monocyte cell types showed any transdifferentiation characteristics under the tested circumstances. Based on our data, we rather see an activation and maturation of monocytes towards macrophages and osteoclasts. The Scientific World Journal 2012-04-30 /pmc/articles/PMC3353470/ /pubmed/22623892 http://dx.doi.org/10.1100/2012/384936 Text en Copyright © 2012 Andreas Schmitt et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Schmitt, Andreas Ehnert, Sabrina Schyschka, Lilianna Buschner, Peter Kühnl, Andreas Döbele, Stefan Siebenlist, Sebastian Lucke, Martin Stöckle, Ulrich Nussler, Andreas K. Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title_full | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title_fullStr | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title_full_unstemmed | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title_short | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
title_sort | monocytes do not transdifferentiate into proper osteoblasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353470/ https://www.ncbi.nlm.nih.gov/pubmed/22623892 http://dx.doi.org/10.1100/2012/384936 |
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