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Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices

While many natural and synthetic compounds exhibit auxin-like activity in bioassays, indole-3-acetic acid (IAA) is recognized as the key auxin in most plants. IAA has been implicated in almost all aspects of plant growth and development and a large array of bacteria have been reported to enhance pla...

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Autores principales: Celloto, Valéria R., Oliveira, Arildo J. B., Gonçalves, José E., Watanabe, Cecília S. F., Matioli, Graciette, Gonçalves, Regina A. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353478/
https://www.ncbi.nlm.nih.gov/pubmed/22623901
http://dx.doi.org/10.1100/2012/495970
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author Celloto, Valéria R.
Oliveira, Arildo J. B.
Gonçalves, José E.
Watanabe, Cecília S. F.
Matioli, Graciette
Gonçalves, Regina A. C.
author_facet Celloto, Valéria R.
Oliveira, Arildo J. B.
Gonçalves, José E.
Watanabe, Cecília S. F.
Matioli, Graciette
Gonçalves, Regina A. C.
author_sort Celloto, Valéria R.
collection PubMed
description While many natural and synthetic compounds exhibit auxin-like activity in bioassays, indole-3-acetic acid (IAA) is recognized as the key auxin in most plants. IAA has been implicated in almost all aspects of plant growth and development and a large array of bacteria have been reported to enhance plant growth. Cells of Klebsiella oxytoca isolated from the rhizosphere of Aspidosperma polyneuron and immobilized by adsorption on different inorganic matrices were used for IAA production. The matrices were prepared by the sol-gel method and the silica-titanium was the most suitable matrix for effective immobilization. In operational stability assays, IAA production was maintained after four cycles of production, obtaining 42.80 ± 2.03 μg mL(−1) of IAA in the third cycle, which corresponds to a 54% increase in production in relation to the first cycle, whereas free cells began losing activity after the first cycle. After 90 days of storage at 4°C the immobilized cells showed the slight reduction of IAA production without significant loss of activity.
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spelling pubmed-33534782012-05-23 Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices Celloto, Valéria R. Oliveira, Arildo J. B. Gonçalves, José E. Watanabe, Cecília S. F. Matioli, Graciette Gonçalves, Regina A. C. ScientificWorldJournal Research Article While many natural and synthetic compounds exhibit auxin-like activity in bioassays, indole-3-acetic acid (IAA) is recognized as the key auxin in most plants. IAA has been implicated in almost all aspects of plant growth and development and a large array of bacteria have been reported to enhance plant growth. Cells of Klebsiella oxytoca isolated from the rhizosphere of Aspidosperma polyneuron and immobilized by adsorption on different inorganic matrices were used for IAA production. The matrices were prepared by the sol-gel method and the silica-titanium was the most suitable matrix for effective immobilization. In operational stability assays, IAA production was maintained after four cycles of production, obtaining 42.80 ± 2.03 μg mL(−1) of IAA in the third cycle, which corresponds to a 54% increase in production in relation to the first cycle, whereas free cells began losing activity after the first cycle. After 90 days of storage at 4°C the immobilized cells showed the slight reduction of IAA production without significant loss of activity. The Scientific World Journal 2012-05-01 /pmc/articles/PMC3353478/ /pubmed/22623901 http://dx.doi.org/10.1100/2012/495970 Text en Copyright © 2012 Valéria R. Celloto et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Celloto, Valéria R.
Oliveira, Arildo J. B.
Gonçalves, José E.
Watanabe, Cecília S. F.
Matioli, Graciette
Gonçalves, Regina A. C.
Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title_full Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title_fullStr Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title_full_unstemmed Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title_short Biosynthesis of Indole-3-Acetic Acid by New Klebsiella oxytoca Free and Immobilized Cells on Inorganic Matrices
title_sort biosynthesis of indole-3-acetic acid by new klebsiella oxytoca free and immobilized cells on inorganic matrices
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353478/
https://www.ncbi.nlm.nih.gov/pubmed/22623901
http://dx.doi.org/10.1100/2012/495970
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