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Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication

Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate...

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Autores principales: Konc, János, Kanyo, Katalin, Kriston, Rita, Zeke, József, Cseh, Sándor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353562/
https://www.ncbi.nlm.nih.gov/pubmed/22629197
http://dx.doi.org/10.1100/2012/785421
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author Konc, János
Kanyo, Katalin
Kriston, Rita
Zeke, József
Cseh, Sándor
author_facet Konc, János
Kanyo, Katalin
Kriston, Rita
Zeke, József
Cseh, Sándor
author_sort Konc, János
collection PubMed
description Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. A total of 143 embryos were transferred into 63 patients resulting in 11 clinical pregnancies (17.5%), 7 of which resulted in live birth of 8 babies (1 twin pregnancy). We were able to detect the spindle in 221 of 259 oocytes (85.3%). After thawing and culturing the oocytes, we were able to visualize the spindle in 177 of 210 oocytes (84.3%). In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position/location relative to the polar body. Our results show that after thawing and culture in half of the spindle-positive oocytes the spindle was detected in a new location, indicating that the spindle and the polar body move relative to each other.
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spelling pubmed-33535622012-05-24 Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication Konc, János Kanyo, Katalin Kriston, Rita Zeke, József Cseh, Sándor ScientificWorldJournal Research Article Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. A total of 143 embryos were transferred into 63 patients resulting in 11 clinical pregnancies (17.5%), 7 of which resulted in live birth of 8 babies (1 twin pregnancy). We were able to detect the spindle in 221 of 259 oocytes (85.3%). After thawing and culturing the oocytes, we were able to visualize the spindle in 177 of 210 oocytes (84.3%). In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position/location relative to the polar body. Our results show that after thawing and culture in half of the spindle-positive oocytes the spindle was detected in a new location, indicating that the spindle and the polar body move relative to each other. The Scientific World Journal 2012-05-02 /pmc/articles/PMC3353562/ /pubmed/22629197 http://dx.doi.org/10.1100/2012/785421 Text en Copyright © 2012 János Konc et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Konc, János
Kanyo, Katalin
Kriston, Rita
Zeke, József
Cseh, Sándor
Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title_full Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title_fullStr Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title_full_unstemmed Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title_short Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
title_sort freezing of oocytes and its effect on the displacement of the meiotic spindle: short communication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353562/
https://www.ncbi.nlm.nih.gov/pubmed/22629197
http://dx.doi.org/10.1100/2012/785421
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