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Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks

The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA) and nonacclimated (NA) stages. Bark tissues subjected to 4°C and −5°C injured to a limited extent in both stages. However, more than 50%...

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Autores principales: Turhan, Ece, Ergin, Sergul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353713/
https://www.ncbi.nlm.nih.gov/pubmed/22629228
http://dx.doi.org/10.1100/2012/979682
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author Turhan, Ece
Ergin, Sergul
author_facet Turhan, Ece
Ergin, Sergul
author_sort Turhan, Ece
collection PubMed
description The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA) and nonacclimated (NA) stages. Bark tissues subjected to 4°C and −5°C injured to a limited extent in both stages. However, more than 50% injury occurred by temperatures equal to or colder than −15°C only in NA period. Total soluble sugar (TSS), reducing sugars, and sucrose contents were higher in CA than those in NA stages in all samples. The activities of acid invertase (EC 3.2.1.26) and sucrose synthase (SS) (EC 2.4.2.13) enzymes were higher in NA stage than those in CA stage. Considering the rootstocks, reducing sugars were higher in both cultivars grafted on Gisela 5 whereas sucrose contents were higher in both cultivars grafted on Mazzard. However, the enzyme activities of both cultivars were higher on Mazzard rootstock than on Gisela 5. In conclusion, cold hardiness of sweet cherry graft combinations was suggested by increasing their TSS, reducing sugars, and sucrose contents significantly in the CA stage. Moreover, acid invertase and SS are down regulated during cold acclimation. Indeed the results suggested that Mazzard is more cold-hardy rootstock than Gisela 5.
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spelling pubmed-33537132012-05-24 Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks Turhan, Ece Ergin, Sergul ScientificWorldJournal Research Article The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA) and nonacclimated (NA) stages. Bark tissues subjected to 4°C and −5°C injured to a limited extent in both stages. However, more than 50% injury occurred by temperatures equal to or colder than −15°C only in NA period. Total soluble sugar (TSS), reducing sugars, and sucrose contents were higher in CA than those in NA stages in all samples. The activities of acid invertase (EC 3.2.1.26) and sucrose synthase (SS) (EC 2.4.2.13) enzymes were higher in NA stage than those in CA stage. Considering the rootstocks, reducing sugars were higher in both cultivars grafted on Gisela 5 whereas sucrose contents were higher in both cultivars grafted on Mazzard. However, the enzyme activities of both cultivars were higher on Mazzard rootstock than on Gisela 5. In conclusion, cold hardiness of sweet cherry graft combinations was suggested by increasing their TSS, reducing sugars, and sucrose contents significantly in the CA stage. Moreover, acid invertase and SS are down regulated during cold acclimation. Indeed the results suggested that Mazzard is more cold-hardy rootstock than Gisela 5. The Scientific World Journal 2012-05-02 /pmc/articles/PMC3353713/ /pubmed/22629228 http://dx.doi.org/10.1100/2012/979682 Text en Copyright © 2012 E. Turhan and S. Ergin. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Turhan, Ece
Ergin, Sergul
Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title_full Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title_fullStr Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title_full_unstemmed Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title_short Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks
title_sort soluble sugars and sucrose-metabolizing enzymes related to cold acclimation of sweet cherry cultivars grafted on different rootstocks
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353713/
https://www.ncbi.nlm.nih.gov/pubmed/22629228
http://dx.doi.org/10.1100/2012/979682
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