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Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe

Receptors of the Eph family of tyrosine kinases and their Ephrin ligands are involved in developmental processes as diverse as angiogenesis, axon guidance and cell migration. However, our understanding of the Eph signaling pathway is incomplete, and could benefit from an analysis by genetic methods....

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Autores principales: Dearborn, Richard E., Dai, Yong, Reed, Brian, Karian, Tamar, Gray, Jessica, Kunes, Sam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353934/
https://www.ncbi.nlm.nih.gov/pubmed/22615969
http://dx.doi.org/10.1371/journal.pone.0037303
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author Dearborn, Richard E.
Dai, Yong
Reed, Brian
Karian, Tamar
Gray, Jessica
Kunes, Sam
author_facet Dearborn, Richard E.
Dai, Yong
Reed, Brian
Karian, Tamar
Gray, Jessica
Kunes, Sam
author_sort Dearborn, Richard E.
collection PubMed
description Receptors of the Eph family of tyrosine kinases and their Ephrin ligands are involved in developmental processes as diverse as angiogenesis, axon guidance and cell migration. However, our understanding of the Eph signaling pathway is incomplete, and could benefit from an analysis by genetic methods. To this end, we performed a genetic modifier screen for mutations that affect Eph signaling in Drosophila melanogaster. Several dozen loci were identified on the basis of their suppression or enhancement of an eye defect induced by the ectopic expression of Ephrin during development; many of these mutant loci were found to disrupt visual system development. One modifier locus, reph (regulator of eph expression), was characterized in molecular detail and found to encode a putative nuclear protein that interacts genetically with Eph signaling pathway mutations. Reph is an autonomous regulator of Eph receptor expression, required for the graded expression of Eph protein and the establishment of an optic lobe axonal topographic map. These results reveal a novel component of the regulatory pathway controlling expression of eph and identify reph as a novel factor in the developing visual system.
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spelling pubmed-33539342012-05-21 Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe Dearborn, Richard E. Dai, Yong Reed, Brian Karian, Tamar Gray, Jessica Kunes, Sam PLoS One Research Article Receptors of the Eph family of tyrosine kinases and their Ephrin ligands are involved in developmental processes as diverse as angiogenesis, axon guidance and cell migration. However, our understanding of the Eph signaling pathway is incomplete, and could benefit from an analysis by genetic methods. To this end, we performed a genetic modifier screen for mutations that affect Eph signaling in Drosophila melanogaster. Several dozen loci were identified on the basis of their suppression or enhancement of an eye defect induced by the ectopic expression of Ephrin during development; many of these mutant loci were found to disrupt visual system development. One modifier locus, reph (regulator of eph expression), was characterized in molecular detail and found to encode a putative nuclear protein that interacts genetically with Eph signaling pathway mutations. Reph is an autonomous regulator of Eph receptor expression, required for the graded expression of Eph protein and the establishment of an optic lobe axonal topographic map. These results reveal a novel component of the regulatory pathway controlling expression of eph and identify reph as a novel factor in the developing visual system. Public Library of Science 2012-05-16 /pmc/articles/PMC3353934/ /pubmed/22615969 http://dx.doi.org/10.1371/journal.pone.0037303 Text en Dearborn et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dearborn, Richard E.
Dai, Yong
Reed, Brian
Karian, Tamar
Gray, Jessica
Kunes, Sam
Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title_full Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title_fullStr Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title_full_unstemmed Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title_short Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
title_sort reph, a regulator of eph receptor expression in the drosophila melanogaster optic lobe
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353934/
https://www.ncbi.nlm.nih.gov/pubmed/22615969
http://dx.doi.org/10.1371/journal.pone.0037303
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