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Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1

The 22Rv1 cell line is widely used for prostate cancer research and other studies throughout the world. These cells were established from a human prostate tumor, CWR22, that was serially passaged in nude mice and selected for androgen independence. The 22Rv1 cells are known to produce high titers of...

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Autores principales: Das Gupta, Jaydip, Luk, Ka-Cheung, Tang, Ning, Gaughan, Christina, Klein, Eric A., Kandel, Eugene S., Hackett, John, Silverman, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353988/
https://www.ncbi.nlm.nih.gov/pubmed/22615748
http://dx.doi.org/10.1371/journal.pone.0036072
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author Das Gupta, Jaydip
Luk, Ka-Cheung
Tang, Ning
Gaughan, Christina
Klein, Eric A.
Kandel, Eugene S.
Hackett, John
Silverman, Robert H.
author_facet Das Gupta, Jaydip
Luk, Ka-Cheung
Tang, Ning
Gaughan, Christina
Klein, Eric A.
Kandel, Eugene S.
Hackett, John
Silverman, Robert H.
author_sort Das Gupta, Jaydip
collection PubMed
description The 22Rv1 cell line is widely used for prostate cancer research and other studies throughout the world. These cells were established from a human prostate tumor, CWR22, that was serially passaged in nude mice and selected for androgen independence. The 22Rv1 cells are known to produce high titers of xenotropic murine leukemia virus-related virus (XMRV). Recent studies suggested that XMRV was inadvertently created in the 1990's when two murine leukemia virus (MLV) genomes (pre-XMRV1 and pre-XMRV-2) recombined during passaging of the CWR22 tumor in mice. The conclusion that XMRV originated from mice and not the patient was based partly on the failure to detect XMRV in early CWR22 xenografts. While that deduction is certainly justified, we examined the possibility that a closely related virus could have been present in primary tumor tissue. Here we report that we have located the original prostate tumor tissue excised from patient CWR22 and have assayed the corresponding DNA by PCR and the tissue sections by fluorescence in situ hybridization for the presence of XMRV or a similar virus. The primary tumor tissues lacked mouse DNA as determined by PCR for intracisternal A type particle DNA, thus avoiding one of the limitations of studying xenografts. We show that neither XMRV nor a closely related virus was present in primary prostate tissue of patient CWR22. Our findings confirm and reinforce the conclusion that XMRV is a recombinant laboratory-generated mouse virus that is highly adapted for human prostate cancer cells.
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spelling pubmed-33539882012-05-21 Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1 Das Gupta, Jaydip Luk, Ka-Cheung Tang, Ning Gaughan, Christina Klein, Eric A. Kandel, Eugene S. Hackett, John Silverman, Robert H. PLoS One Research Article The 22Rv1 cell line is widely used for prostate cancer research and other studies throughout the world. These cells were established from a human prostate tumor, CWR22, that was serially passaged in nude mice and selected for androgen independence. The 22Rv1 cells are known to produce high titers of xenotropic murine leukemia virus-related virus (XMRV). Recent studies suggested that XMRV was inadvertently created in the 1990's when two murine leukemia virus (MLV) genomes (pre-XMRV1 and pre-XMRV-2) recombined during passaging of the CWR22 tumor in mice. The conclusion that XMRV originated from mice and not the patient was based partly on the failure to detect XMRV in early CWR22 xenografts. While that deduction is certainly justified, we examined the possibility that a closely related virus could have been present in primary tumor tissue. Here we report that we have located the original prostate tumor tissue excised from patient CWR22 and have assayed the corresponding DNA by PCR and the tissue sections by fluorescence in situ hybridization for the presence of XMRV or a similar virus. The primary tumor tissues lacked mouse DNA as determined by PCR for intracisternal A type particle DNA, thus avoiding one of the limitations of studying xenografts. We show that neither XMRV nor a closely related virus was present in primary prostate tissue of patient CWR22. Our findings confirm and reinforce the conclusion that XMRV is a recombinant laboratory-generated mouse virus that is highly adapted for human prostate cancer cells. Public Library of Science 2012-05-16 /pmc/articles/PMC3353988/ /pubmed/22615748 http://dx.doi.org/10.1371/journal.pone.0036072 Text en Das Gupta et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Das Gupta, Jaydip
Luk, Ka-Cheung
Tang, Ning
Gaughan, Christina
Klein, Eric A.
Kandel, Eugene S.
Hackett, John
Silverman, Robert H.
Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title_full Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title_fullStr Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title_full_unstemmed Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title_short Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1
title_sort absence of xmrv and closely related viruses in primary prostate cancer tissues used to derive the xmrv-infected cell line 22rv1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353988/
https://www.ncbi.nlm.nih.gov/pubmed/22615748
http://dx.doi.org/10.1371/journal.pone.0036072
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