Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are kerati...

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Detalles Bibliográficos
Autores principales: Mikulíková, Kateřina, Romanov, Oleg, Miksik, Ivan, Eckhardt, Adam, Pataridis, Statis, Sedláková, Pavla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354689/
https://www.ncbi.nlm.nih.gov/pubmed/22629195
http://dx.doi.org/10.1100/2012/759604
Descripción
Sumario:The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α (1)) (from bovine) and collagen type I (α (2)) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.

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