Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are kerati...

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Detalles Bibliográficos
Autores principales: Mikulíková, Kateřina, Romanov, Oleg, Miksik, Ivan, Eckhardt, Adam, Pataridis, Statis, Sedláková, Pavla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354689/
https://www.ncbi.nlm.nih.gov/pubmed/22629195
http://dx.doi.org/10.1100/2012/759604
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author Mikulíková, Kateřina
Romanov, Oleg
Miksik, Ivan
Eckhardt, Adam
Pataridis, Statis
Sedláková, Pavla
author_facet Mikulíková, Kateřina
Romanov, Oleg
Miksik, Ivan
Eckhardt, Adam
Pataridis, Statis
Sedláková, Pavla
author_sort Mikulíková, Kateřina
collection PubMed
description The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α (1)) (from bovine) and collagen type I (α (2)) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.
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spelling pubmed-33546892012-05-24 Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry Mikulíková, Kateřina Romanov, Oleg Miksik, Ivan Eckhardt, Adam Pataridis, Statis Sedláková, Pavla ScientificWorldJournal Research Article The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α (1)) (from bovine) and collagen type I (α (2)) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme. The Scientific World Journal 2012-05-02 /pmc/articles/PMC3354689/ /pubmed/22629195 http://dx.doi.org/10.1100/2012/759604 Text en Copyright © 2012 Kateřina Mikulíková et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mikulíková, Kateřina
Romanov, Oleg
Miksik, Ivan
Eckhardt, Adam
Pataridis, Statis
Sedláková, Pavla
Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_full Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_fullStr Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_full_unstemmed Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_short Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_sort study of saiga horn using high-performance liquid chromatography with mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354689/
https://www.ncbi.nlm.nih.gov/pubmed/22629195
http://dx.doi.org/10.1100/2012/759604
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