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Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes

The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration...

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Detalles Bibliográficos
Autores principales: Li, Guiling, Fan, Chao, Li, Xinru, Fan, Yating, Wang, Xiaoning, Li, Mei, Liu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354752/
https://www.ncbi.nlm.nih.gov/pubmed/22629219
http://dx.doi.org/10.1100/2012/874053
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author Li, Guiling
Fan, Chao
Li, Xinru
Fan, Yating
Wang, Xiaoning
Li, Mei
Liu, Yan
author_facet Li, Guiling
Fan, Chao
Li, Xinru
Fan, Yating
Wang, Xiaoning
Li, Mei
Liu, Yan
author_sort Li, Guiling
collection PubMed
description The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD). Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4°C). Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD.
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spelling pubmed-33547522012-05-24 Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes Li, Guiling Fan, Chao Li, Xinru Fan, Yating Wang, Xiaoning Li, Mei Liu, Yan ScientificWorldJournal Research Article The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD). Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4°C). Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD. The Scientific World Journal 2012-05-02 /pmc/articles/PMC3354752/ /pubmed/22629219 http://dx.doi.org/10.1100/2012/874053 Text en Copyright © 2012 Guiling Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Guiling
Fan, Chao
Li, Xinru
Fan, Yating
Wang, Xiaoning
Li, Mei
Liu, Yan
Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title_full Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title_fullStr Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title_full_unstemmed Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title_short Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
title_sort preparation and in vitro evaluation of tacrolimus-loaded ethosomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354752/
https://www.ncbi.nlm.nih.gov/pubmed/22629219
http://dx.doi.org/10.1100/2012/874053
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