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The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation
The study of metabolic regulation has traditionally focused on analysis of specific enzymes, emphasizing kinetic properties, and the influence of protein interactions and post-translational modifications. More recently, reverse genetic approaches permit researchers to directly determine the effects...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3355787/ https://www.ncbi.nlm.nih.gov/pubmed/22645543 http://dx.doi.org/10.3389/fpls.2011.00059 |
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author | Fernie, Alisdair R. Klee, Harry J. |
author_facet | Fernie, Alisdair R. Klee, Harry J. |
author_sort | Fernie, Alisdair R. |
collection | PubMed |
description | The study of metabolic regulation has traditionally focused on analysis of specific enzymes, emphasizing kinetic properties, and the influence of protein interactions and post-translational modifications. More recently, reverse genetic approaches permit researchers to directly determine the effects of a deficiency or a surplus of a given enzyme on the biochemistry and physiology of a plant. Furthermore, in many model species, gene expression atlases that give important spatial information concerning the quantitative expression level of metabolism-associated genes are being produced. In parallel, “top-down” approaches to understand metabolic regulation have recently been instigated whereby broad genetic diversity is screened for metabolic traits and the genetic basis of this diversity is defined thereafter. In this article we will review recent examples of this latter approach both in the model species Arabidopsis thaliana and the crop species tomato (Solanum lycopersicum). In addition to highlighting examples in which this genetic diversity approach has proven promising, we will discuss the challenges associated with this approach and provide a perspective for its future utility. |
format | Online Article Text |
id | pubmed-3355787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33557872012-05-29 The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation Fernie, Alisdair R. Klee, Harry J. Front Plant Sci Plant Science The study of metabolic regulation has traditionally focused on analysis of specific enzymes, emphasizing kinetic properties, and the influence of protein interactions and post-translational modifications. More recently, reverse genetic approaches permit researchers to directly determine the effects of a deficiency or a surplus of a given enzyme on the biochemistry and physiology of a plant. Furthermore, in many model species, gene expression atlases that give important spatial information concerning the quantitative expression level of metabolism-associated genes are being produced. In parallel, “top-down” approaches to understand metabolic regulation have recently been instigated whereby broad genetic diversity is screened for metabolic traits and the genetic basis of this diversity is defined thereafter. In this article we will review recent examples of this latter approach both in the model species Arabidopsis thaliana and the crop species tomato (Solanum lycopersicum). In addition to highlighting examples in which this genetic diversity approach has proven promising, we will discuss the challenges associated with this approach and provide a perspective for its future utility. Frontiers Research Foundation 2011-09-30 /pmc/articles/PMC3355787/ /pubmed/22645543 http://dx.doi.org/10.3389/fpls.2011.00059 Text en Copyright © 2011 Fernie and Klee. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with. |
spellingShingle | Plant Science Fernie, Alisdair R. Klee, Harry J. The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title | The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title_full | The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title_fullStr | The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title_full_unstemmed | The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title_short | The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation |
title_sort | use of natural genetic diversity in the understanding of metabolic organization and regulation |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3355787/ https://www.ncbi.nlm.nih.gov/pubmed/22645543 http://dx.doi.org/10.3389/fpls.2011.00059 |
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