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Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficie...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356165/ https://www.ncbi.nlm.nih.gov/pubmed/22619530 http://dx.doi.org/10.2147/IJN.S29958 |
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author | Bei, Yong-yan Chen, Xiao-yan Liu, Yang Xu, Jing-yu Wang, Wen-juan Gu, Zong-lin Xing, Kong-lang Zhu, Ai-jun Chen, Wei-liang Shi, Lin-seng Wang, Qin Zhang, Xue-nong Zhang, Qiang |
author_facet | Bei, Yong-yan Chen, Xiao-yan Liu, Yang Xu, Jing-yu Wang, Wen-juan Gu, Zong-lin Xing, Kong-lang Zhu, Ai-jun Chen, Wei-liang Shi, Lin-seng Wang, Qin Zhang, Xue-nong Zhang, Qiang |
author_sort | Bei, Yong-yan |
collection | PubMed |
description | In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficiency, and drug release characteristics of the nanoparticles were investigated in vitro. To investigate the intestinal absorption mechanisms of the two preparations, a series of experiments was carried out, including in situ circulation method, in vitro everted gut sacs, and Ussing chamber perfusion technique. The absorption rate constants (Ka) of NCTD at different segments were found to be duodenum > jejunum > ileum > colon. The concentration had no distinctive effect on absorption kinetics, suggesting that drug absorption is not dose-dependent. The transport of NCTD was found to be inhibited by P-glycoprotein (P-gp) inhibitor, indicating that NCTD might be the substrate of P-gp. The order of the absorption enhancer effects were as follows: low molecular weight chitosan (CS-8kDa) > high molecular weight chitosan (CS-30kDa) > Poloxamer > sodium dodecyl sulfate (SDS) > sodium deoxycholate (SDCh). The results indicate that the chitosan nanoparticles can improve intestinal absorption of NCTD. |
format | Online Article Text |
id | pubmed-3356165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33561652012-05-22 Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption Bei, Yong-yan Chen, Xiao-yan Liu, Yang Xu, Jing-yu Wang, Wen-juan Gu, Zong-lin Xing, Kong-lang Zhu, Ai-jun Chen, Wei-liang Shi, Lin-seng Wang, Qin Zhang, Xue-nong Zhang, Qiang Int J Nanomedicine Original Research In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficiency, and drug release characteristics of the nanoparticles were investigated in vitro. To investigate the intestinal absorption mechanisms of the two preparations, a series of experiments was carried out, including in situ circulation method, in vitro everted gut sacs, and Ussing chamber perfusion technique. The absorption rate constants (Ka) of NCTD at different segments were found to be duodenum > jejunum > ileum > colon. The concentration had no distinctive effect on absorption kinetics, suggesting that drug absorption is not dose-dependent. The transport of NCTD was found to be inhibited by P-glycoprotein (P-gp) inhibitor, indicating that NCTD might be the substrate of P-gp. The order of the absorption enhancer effects were as follows: low molecular weight chitosan (CS-8kDa) > high molecular weight chitosan (CS-30kDa) > Poloxamer > sodium dodecyl sulfate (SDS) > sodium deoxycholate (SDCh). The results indicate that the chitosan nanoparticles can improve intestinal absorption of NCTD. Dove Medical Press 2012 2012-04-03 /pmc/articles/PMC3356165/ /pubmed/22619530 http://dx.doi.org/10.2147/IJN.S29958 Text en © 2012 Bei et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Bei, Yong-yan Chen, Xiao-yan Liu, Yang Xu, Jing-yu Wang, Wen-juan Gu, Zong-lin Xing, Kong-lang Zhu, Ai-jun Chen, Wei-liang Shi, Lin-seng Wang, Qin Zhang, Xue-nong Zhang, Qiang Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title | Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title_full | Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title_fullStr | Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title_full_unstemmed | Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title_short | Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
title_sort | novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356165/ https://www.ncbi.nlm.nih.gov/pubmed/22619530 http://dx.doi.org/10.2147/IJN.S29958 |
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