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Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption

In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficie...

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Detalles Bibliográficos
Autores principales: Bei, Yong-yan, Chen, Xiao-yan, Liu, Yang, Xu, Jing-yu, Wang, Wen-juan, Gu, Zong-lin, Xing, Kong-lang, Zhu, Ai-jun, Chen, Wei-liang, Shi, Lin-seng, Wang, Qin, Zhang, Xue-nong, Zhang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356165/
https://www.ncbi.nlm.nih.gov/pubmed/22619530
http://dx.doi.org/10.2147/IJN.S29958
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author Bei, Yong-yan
Chen, Xiao-yan
Liu, Yang
Xu, Jing-yu
Wang, Wen-juan
Gu, Zong-lin
Xing, Kong-lang
Zhu, Ai-jun
Chen, Wei-liang
Shi, Lin-seng
Wang, Qin
Zhang, Xue-nong
Zhang, Qiang
author_facet Bei, Yong-yan
Chen, Xiao-yan
Liu, Yang
Xu, Jing-yu
Wang, Wen-juan
Gu, Zong-lin
Xing, Kong-lang
Zhu, Ai-jun
Chen, Wei-liang
Shi, Lin-seng
Wang, Qin
Zhang, Xue-nong
Zhang, Qiang
author_sort Bei, Yong-yan
collection PubMed
description In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficiency, and drug release characteristics of the nanoparticles were investigated in vitro. To investigate the intestinal absorption mechanisms of the two preparations, a series of experiments was carried out, including in situ circulation method, in vitro everted gut sacs, and Ussing chamber perfusion technique. The absorption rate constants (Ka) of NCTD at different segments were found to be duodenum > jejunum > ileum > colon. The concentration had no distinctive effect on absorption kinetics, suggesting that drug absorption is not dose-dependent. The transport of NCTD was found to be inhibited by P-glycoprotein (P-gp) inhibitor, indicating that NCTD might be the substrate of P-gp. The order of the absorption enhancer effects were as follows: low molecular weight chitosan (CS-8kDa) > high molecular weight chitosan (CS-30kDa) > Poloxamer > sodium dodecyl sulfate (SDS) > sodium deoxycholate (SDCh). The results indicate that the chitosan nanoparticles can improve intestinal absorption of NCTD.
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spelling pubmed-33561652012-05-22 Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption Bei, Yong-yan Chen, Xiao-yan Liu, Yang Xu, Jing-yu Wang, Wen-juan Gu, Zong-lin Xing, Kong-lang Zhu, Ai-jun Chen, Wei-liang Shi, Lin-seng Wang, Qin Zhang, Xue-nong Zhang, Qiang Int J Nanomedicine Original Research In this paper, two novel liver-targeting nanoparticles, norcantharidin-loaded chitosan nanoparticles (NCTD-CS-NPs) and norcantharidin-associated galactosylated chitosan nanoparticles (NCTD-GC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, encapsulation efficiency, and drug release characteristics of the nanoparticles were investigated in vitro. To investigate the intestinal absorption mechanisms of the two preparations, a series of experiments was carried out, including in situ circulation method, in vitro everted gut sacs, and Ussing chamber perfusion technique. The absorption rate constants (Ka) of NCTD at different segments were found to be duodenum > jejunum > ileum > colon. The concentration had no distinctive effect on absorption kinetics, suggesting that drug absorption is not dose-dependent. The transport of NCTD was found to be inhibited by P-glycoprotein (P-gp) inhibitor, indicating that NCTD might be the substrate of P-gp. The order of the absorption enhancer effects were as follows: low molecular weight chitosan (CS-8kDa) > high molecular weight chitosan (CS-30kDa) > Poloxamer > sodium dodecyl sulfate (SDS) > sodium deoxycholate (SDCh). The results indicate that the chitosan nanoparticles can improve intestinal absorption of NCTD. Dove Medical Press 2012 2012-04-03 /pmc/articles/PMC3356165/ /pubmed/22619530 http://dx.doi.org/10.2147/IJN.S29958 Text en © 2012 Bei et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Bei, Yong-yan
Chen, Xiao-yan
Liu, Yang
Xu, Jing-yu
Wang, Wen-juan
Gu, Zong-lin
Xing, Kong-lang
Zhu, Ai-jun
Chen, Wei-liang
Shi, Lin-seng
Wang, Qin
Zhang, Xue-nong
Zhang, Qiang
Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title_full Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title_fullStr Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title_full_unstemmed Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title_short Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
title_sort novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356165/
https://www.ncbi.nlm.nih.gov/pubmed/22619530
http://dx.doi.org/10.2147/IJN.S29958
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