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Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging

Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300–325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of eur...

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Autores principales: Avti, Pramod K, Sitharaman, Balaji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356200/
https://www.ncbi.nlm.nih.gov/pubmed/22619533
http://dx.doi.org/10.2147/IJN.S29545
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author Avti, Pramod K
Sitharaman, Balaji
author_facet Avti, Pramod K
Sitharaman, Balaji
author_sort Avti, Pramod K
collection PubMed
description Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300–325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 μg/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%–100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu(3+) ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging.
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spelling pubmed-33562002012-05-22 Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging Avti, Pramod K Sitharaman, Balaji Int J Nanomedicine Original Research Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300–325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 μg/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%–100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu(3+) ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging. Dove Medical Press 2012 2012-04-17 /pmc/articles/PMC3356200/ /pubmed/22619533 http://dx.doi.org/10.2147/IJN.S29545 Text en © 2012 Avti and Sitharaman, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Avti, Pramod K
Sitharaman, Balaji
Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_full Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_fullStr Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_full_unstemmed Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_short Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_sort luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356200/
https://www.ncbi.nlm.nih.gov/pubmed/22619533
http://dx.doi.org/10.2147/IJN.S29545
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