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Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel c...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356208/ https://www.ncbi.nlm.nih.gov/pubmed/22619539 http://dx.doi.org/10.2147/IJN.S30928 |
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author | Jiang, Zhaoshun Sun, Cong Yin, Zhaohui Zhou, Fang Ge, Linfu Liu, Ximin Kong, Fansheng |
author_facet | Jiang, Zhaoshun Sun, Cong Yin, Zhaohui Zhou, Fang Ge, Linfu Liu, Ximin Kong, Fansheng |
author_sort | Jiang, Zhaoshun |
collection | PubMed |
description | BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE). METHODS: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages. RESULTS: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo. CONCLUSION: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research. |
format | Online Article Text |
id | pubmed-3356208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33562082012-05-22 Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems Jiang, Zhaoshun Sun, Cong Yin, Zhaohui Zhou, Fang Ge, Linfu Liu, Ximin Kong, Fansheng Int J Nanomedicine Original Research BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE). METHODS: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages. RESULTS: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo. CONCLUSION: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research. Dove Medical Press 2012 2012-04-17 /pmc/articles/PMC3356208/ /pubmed/22619539 http://dx.doi.org/10.2147/IJN.S30928 Text en © 2012 Jiang et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Jiang, Zhaoshun Sun, Cong Yin, Zhaohui Zhou, Fang Ge, Linfu Liu, Ximin Kong, Fansheng Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title | Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title_full | Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title_fullStr | Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title_full_unstemmed | Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title_short | Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
title_sort | comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356208/ https://www.ncbi.nlm.nih.gov/pubmed/22619539 http://dx.doi.org/10.2147/IJN.S30928 |
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