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Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems

BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel c...

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Detalles Bibliográficos
Autores principales: Jiang, Zhaoshun, Sun, Cong, Yin, Zhaohui, Zhou, Fang, Ge, Linfu, Liu, Ximin, Kong, Fansheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356208/
https://www.ncbi.nlm.nih.gov/pubmed/22619539
http://dx.doi.org/10.2147/IJN.S30928
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author Jiang, Zhaoshun
Sun, Cong
Yin, Zhaohui
Zhou, Fang
Ge, Linfu
Liu, Ximin
Kong, Fansheng
author_facet Jiang, Zhaoshun
Sun, Cong
Yin, Zhaohui
Zhou, Fang
Ge, Linfu
Liu, Ximin
Kong, Fansheng
author_sort Jiang, Zhaoshun
collection PubMed
description BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE). METHODS: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages. RESULTS: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo. CONCLUSION: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research.
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spelling pubmed-33562082012-05-22 Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems Jiang, Zhaoshun Sun, Cong Yin, Zhaohui Zhou, Fang Ge, Linfu Liu, Ximin Kong, Fansheng Int J Nanomedicine Original Research BACKGROUND: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE). METHODS: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages. RESULTS: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo. CONCLUSION: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research. Dove Medical Press 2012 2012-04-17 /pmc/articles/PMC3356208/ /pubmed/22619539 http://dx.doi.org/10.2147/IJN.S30928 Text en © 2012 Jiang et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Jiang, Zhaoshun
Sun, Cong
Yin, Zhaohui
Zhou, Fang
Ge, Linfu
Liu, Ximin
Kong, Fansheng
Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_full Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_fullStr Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_full_unstemmed Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_short Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_sort comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356208/
https://www.ncbi.nlm.nih.gov/pubmed/22619539
http://dx.doi.org/10.2147/IJN.S30928
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