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Automated Solid-Phase Subcloning Based on Beads Brought into Proximity by Magnetic Force

In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate construction of expression vectors and genetic pathways. Here, we describe a new approach for solid-phase cloning in which both the vector and the g...

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Detalles Bibliográficos
Autores principales: Hudson, Elton P., Nikoshkov, Andrej, Uhlen, Mathias, Rockberg, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356258/
https://www.ncbi.nlm.nih.gov/pubmed/22624028
http://dx.doi.org/10.1371/journal.pone.0037429