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Automated Solid-Phase Subcloning Based on Beads Brought into Proximity by Magnetic Force
In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate construction of expression vectors and genetic pathways. Here, we describe a new approach for solid-phase cloning in which both the vector and the g...
Autores principales: | Hudson, Elton P., Nikoshkov, Andrej, Uhlen, Mathias, Rockberg, Johan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356258/ https://www.ncbi.nlm.nih.gov/pubmed/22624028 http://dx.doi.org/10.1371/journal.pone.0037429 |
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