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The RIP140 Gene Is a Transcriptional Target of E2F1

RIP140 is a transcriptional coregulator involved in energy homeostasis and ovulation which is controlled at the transcriptional level by several nuclear receptors. We demonstrate here that RIP140 is a novel target gene of the E2F1 transcription factor. Bioinformatics analysis, gel shift assay, and c...

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Autores principales: Docquier, Aurélie, Augereau, Patrick, Lapierre, Marion, Harmand, Pierre-Olivier, Badia, Eric, Annicotte, Jean-Sébastien, Fajas, Lluis, Cavaillès, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356364/
https://www.ncbi.nlm.nih.gov/pubmed/22629304
http://dx.doi.org/10.1371/journal.pone.0035839
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author Docquier, Aurélie
Augereau, Patrick
Lapierre, Marion
Harmand, Pierre-Olivier
Badia, Eric
Annicotte, Jean-Sébastien
Fajas, Lluis
Cavaillès, Vincent
author_facet Docquier, Aurélie
Augereau, Patrick
Lapierre, Marion
Harmand, Pierre-Olivier
Badia, Eric
Annicotte, Jean-Sébastien
Fajas, Lluis
Cavaillès, Vincent
author_sort Docquier, Aurélie
collection PubMed
description RIP140 is a transcriptional coregulator involved in energy homeostasis and ovulation which is controlled at the transcriptional level by several nuclear receptors. We demonstrate here that RIP140 is a novel target gene of the E2F1 transcription factor. Bioinformatics analysis, gel shift assay, and chromatin immunoprecipitation demonstrate that the RIP140 promoter contains bona fide E2F response elements. In transiently transfected MCF-7 breast cancer cells, the RIP140 promoter is transactivated by overexpression of E2F1/DP1. Interestingly, RIP140 mRNA is finely regulated during cell cycle progression (5-fold increase at the G1/S and G2/M transitions). The positive regulation by E2F1 requires sequences located in the proximal region of the promoter (−73/+167), involves Sp1 transcription factors, and undergoes a negative feedback control by RIP140. Finally, we show that E2F1 participates in the induction of RIP140 expression during adipocyte differentiation. Altogether, this work identifies the RIP140 gene as a new transcriptional target of E2F1 which may explain some of the effect of E2F1 in both cancer and metabolic diseases.
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spelling pubmed-33563642012-05-24 The RIP140 Gene Is a Transcriptional Target of E2F1 Docquier, Aurélie Augereau, Patrick Lapierre, Marion Harmand, Pierre-Olivier Badia, Eric Annicotte, Jean-Sébastien Fajas, Lluis Cavaillès, Vincent PLoS One Research Article RIP140 is a transcriptional coregulator involved in energy homeostasis and ovulation which is controlled at the transcriptional level by several nuclear receptors. We demonstrate here that RIP140 is a novel target gene of the E2F1 transcription factor. Bioinformatics analysis, gel shift assay, and chromatin immunoprecipitation demonstrate that the RIP140 promoter contains bona fide E2F response elements. In transiently transfected MCF-7 breast cancer cells, the RIP140 promoter is transactivated by overexpression of E2F1/DP1. Interestingly, RIP140 mRNA is finely regulated during cell cycle progression (5-fold increase at the G1/S and G2/M transitions). The positive regulation by E2F1 requires sequences located in the proximal region of the promoter (−73/+167), involves Sp1 transcription factors, and undergoes a negative feedback control by RIP140. Finally, we show that E2F1 participates in the induction of RIP140 expression during adipocyte differentiation. Altogether, this work identifies the RIP140 gene as a new transcriptional target of E2F1 which may explain some of the effect of E2F1 in both cancer and metabolic diseases. Public Library of Science 2012-05-18 /pmc/articles/PMC3356364/ /pubmed/22629304 http://dx.doi.org/10.1371/journal.pone.0035839 Text en Docquier et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Docquier, Aurélie
Augereau, Patrick
Lapierre, Marion
Harmand, Pierre-Olivier
Badia, Eric
Annicotte, Jean-Sébastien
Fajas, Lluis
Cavaillès, Vincent
The RIP140 Gene Is a Transcriptional Target of E2F1
title The RIP140 Gene Is a Transcriptional Target of E2F1
title_full The RIP140 Gene Is a Transcriptional Target of E2F1
title_fullStr The RIP140 Gene Is a Transcriptional Target of E2F1
title_full_unstemmed The RIP140 Gene Is a Transcriptional Target of E2F1
title_short The RIP140 Gene Is a Transcriptional Target of E2F1
title_sort rip140 gene is a transcriptional target of e2f1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356364/
https://www.ncbi.nlm.nih.gov/pubmed/22629304
http://dx.doi.org/10.1371/journal.pone.0035839
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