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Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice

MicroRNAs interact with multiple mRNAs resulting in their degradation and/or translational repression. This report used the delayed implantation model to determine the role of miRNAs in blastocysts. Dormant blastocysts in delayed implanting mice were activated by estradiol. Differential expression o...

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Autores principales: Liu, Wei-Min, Pang, Ronald T. K., Cheong, Ana W. Y., Ng, Ernest H. Y., Lao, Kaiqin, Lee, Kai-Fai, Yeung, William S. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356405/
https://www.ncbi.nlm.nih.gov/pubmed/22623977
http://dx.doi.org/10.1371/journal.pone.0037039
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author Liu, Wei-Min
Pang, Ronald T. K.
Cheong, Ana W. Y.
Ng, Ernest H. Y.
Lao, Kaiqin
Lee, Kai-Fai
Yeung, William S. B.
author_facet Liu, Wei-Min
Pang, Ronald T. K.
Cheong, Ana W. Y.
Ng, Ernest H. Y.
Lao, Kaiqin
Lee, Kai-Fai
Yeung, William S. B.
author_sort Liu, Wei-Min
collection PubMed
description MicroRNAs interact with multiple mRNAs resulting in their degradation and/or translational repression. This report used the delayed implantation model to determine the role of miRNAs in blastocysts. Dormant blastocysts in delayed implanting mice were activated by estradiol. Differential expression of 45 out of 238 miRNAs examined was found between the dormant and the activated blastocysts. Five of the nine members of the microRNA lethal-7 (let-7) family were down-regulated after activation. Human blastocysts also had a low expression of let-7 family. Forced-expression of a family member, let-7a in mouse blastocysts decreased the number of implantation sites (let-7a: 1.1±0.4; control: 3.8±0.4) in vivo, and reduced the percentages of blastocyst that attached (let-7a: 42.0±8.3%; control: 79.0±5.1%) and spreaded (let-7a: 33.5±2.9%; control: 67.3±3.8%) on fibronectin in vitro. Integrin-β3, a known implantation-related molecule, was demonstrated to be a target of let-7a by 3′-untranslated region reporter assay in cervical cancer cells HeLa, and Western blotting in mouse blastocysts. The inhibitory effect of forced-expression of let-7a on blastocyst attachment and outgrowth was partially nullified in vitro and in vivo by forced-expression of integrin-β3. This study provides the first direct evidence that let-7a is involved in regulating the implantation process partly via modulation of the expression of integrin-β3. (200 words).
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spelling pubmed-33564052012-05-23 Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice Liu, Wei-Min Pang, Ronald T. K. Cheong, Ana W. Y. Ng, Ernest H. Y. Lao, Kaiqin Lee, Kai-Fai Yeung, William S. B. PLoS One Research Article MicroRNAs interact with multiple mRNAs resulting in their degradation and/or translational repression. This report used the delayed implantation model to determine the role of miRNAs in blastocysts. Dormant blastocysts in delayed implanting mice were activated by estradiol. Differential expression of 45 out of 238 miRNAs examined was found between the dormant and the activated blastocysts. Five of the nine members of the microRNA lethal-7 (let-7) family were down-regulated after activation. Human blastocysts also had a low expression of let-7 family. Forced-expression of a family member, let-7a in mouse blastocysts decreased the number of implantation sites (let-7a: 1.1±0.4; control: 3.8±0.4) in vivo, and reduced the percentages of blastocyst that attached (let-7a: 42.0±8.3%; control: 79.0±5.1%) and spreaded (let-7a: 33.5±2.9%; control: 67.3±3.8%) on fibronectin in vitro. Integrin-β3, a known implantation-related molecule, was demonstrated to be a target of let-7a by 3′-untranslated region reporter assay in cervical cancer cells HeLa, and Western blotting in mouse blastocysts. The inhibitory effect of forced-expression of let-7a on blastocyst attachment and outgrowth was partially nullified in vitro and in vivo by forced-expression of integrin-β3. This study provides the first direct evidence that let-7a is involved in regulating the implantation process partly via modulation of the expression of integrin-β3. (200 words). Public Library of Science 2012-05-18 /pmc/articles/PMC3356405/ /pubmed/22623977 http://dx.doi.org/10.1371/journal.pone.0037039 Text en Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Wei-Min
Pang, Ronald T. K.
Cheong, Ana W. Y.
Ng, Ernest H. Y.
Lao, Kaiqin
Lee, Kai-Fai
Yeung, William S. B.
Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title_full Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title_fullStr Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title_full_unstemmed Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title_short Involvement of microRNA Lethal-7a in the Regulation of Embryo Implantation in Mice
title_sort involvement of microrna lethal-7a in the regulation of embryo implantation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356405/
https://www.ncbi.nlm.nih.gov/pubmed/22623977
http://dx.doi.org/10.1371/journal.pone.0037039
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