Cargando…

Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer

Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer “SpiralTOF (STOF)”, a collision cell, and an offset parabolic reflectro...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimma, Shuichi, Kubo, Ayumi, Satoh, Takaya, Toyoda, Michisato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356408/
https://www.ncbi.nlm.nih.gov/pubmed/22623981
http://dx.doi.org/10.1371/journal.pone.0037107
_version_ 1782233562352713728
author Shimma, Shuichi
Kubo, Ayumi
Satoh, Takaya
Toyoda, Michisato
author_facet Shimma, Shuichi
Kubo, Ayumi
Satoh, Takaya
Toyoda, Michisato
author_sort Shimma, Shuichi
collection PubMed
description Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer “SpiralTOF (STOF)”, a collision cell, and an offset parabolic reflectron (RTOF). The features of this system are high precursor ion selectivity due to a 17-m flight path length in STOF and elimination of post-source decay (PSD) ions. The acceleration energy is 20 keV, so that high-energy collision-induced dissociation (HE-CID) is possible. Elimination of PSD ions allows observation of the product ions inherent to the HE-CID process. By using this tandem TOF instrument, the product ion spectrum of lipids provided detailed structural information of fatty acid residues.
format Online
Article
Text
id pubmed-3356408
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-33564082012-05-23 Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer Shimma, Shuichi Kubo, Ayumi Satoh, Takaya Toyoda, Michisato PLoS One Research Article Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer “SpiralTOF (STOF)”, a collision cell, and an offset parabolic reflectron (RTOF). The features of this system are high precursor ion selectivity due to a 17-m flight path length in STOF and elimination of post-source decay (PSD) ions. The acceleration energy is 20 keV, so that high-energy collision-induced dissociation (HE-CID) is possible. Elimination of PSD ions allows observation of the product ions inherent to the HE-CID process. By using this tandem TOF instrument, the product ion spectrum of lipids provided detailed structural information of fatty acid residues. Public Library of Science 2012-05-18 /pmc/articles/PMC3356408/ /pubmed/22623981 http://dx.doi.org/10.1371/journal.pone.0037107 Text en Shimma et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shimma, Shuichi
Kubo, Ayumi
Satoh, Takaya
Toyoda, Michisato
Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title_full Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title_fullStr Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title_full_unstemmed Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title_short Detailed Structural Analysis of Lipids Directly on Tissue Specimens Using a MALDI-SpiralTOF-Reflectron TOF Mass Spectrometer
title_sort detailed structural analysis of lipids directly on tissue specimens using a maldi-spiraltof-reflectron tof mass spectrometer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356408/
https://www.ncbi.nlm.nih.gov/pubmed/22623981
http://dx.doi.org/10.1371/journal.pone.0037107
work_keys_str_mv AT shimmashuichi detailedstructuralanalysisoflipidsdirectlyontissuespecimensusingamaldispiraltofreflectrontofmassspectrometer
AT kuboayumi detailedstructuralanalysisoflipidsdirectlyontissuespecimensusingamaldispiraltofreflectrontofmassspectrometer
AT satohtakaya detailedstructuralanalysisoflipidsdirectlyontissuespecimensusingamaldispiraltofreflectrontofmassspectrometer
AT toyodamichisato detailedstructuralanalysisoflipidsdirectlyontissuespecimensusingamaldispiraltofreflectrontofmassspectrometer