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The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Resul...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357049/ https://www.ncbi.nlm.nih.gov/pubmed/22615529 http://dx.doi.org/10.2147/IJN.S30060 |
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author | Hsieh, Dar-Shih Lu, Hsiu-Chin Chen, Cheng-Cheung Wu, Chang-Jer Yeh, Ming-Kung |
author_facet | Hsieh, Dar-Shih Lu, Hsiu-Chin Chen, Cheng-Cheung Wu, Chang-Jer Yeh, Ming-Kung |
author_sort | Hsieh, Dar-Shih |
collection | PubMed |
description | Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to −22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of −8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities. |
format | Online Article Text |
id | pubmed-3357049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33570492012-05-21 The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system Hsieh, Dar-Shih Lu, Hsiu-Chin Chen, Cheng-Cheung Wu, Chang-Jer Yeh, Ming-Kung Int J Nanomedicine Original Research Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to −22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of −8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities. Dove Medical Press 2012 2012-03-28 /pmc/articles/PMC3357049/ /pubmed/22615529 http://dx.doi.org/10.2147/IJN.S30060 Text en © 2012 Hsieh et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Hsieh, Dar-Shih Lu, Hsiu-Chin Chen, Cheng-Cheung Wu, Chang-Jer Yeh, Ming-Kung The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title | The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title_full | The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title_fullStr | The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title_full_unstemmed | The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title_short | The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
title_sort | preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357049/ https://www.ncbi.nlm.nih.gov/pubmed/22615529 http://dx.doi.org/10.2147/IJN.S30060 |
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