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The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Resul...

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Autores principales: Hsieh, Dar-Shih, Lu, Hsiu-Chin, Chen, Cheng-Cheung, Wu, Chang-Jer, Yeh, Ming-Kung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357049/
https://www.ncbi.nlm.nih.gov/pubmed/22615529
http://dx.doi.org/10.2147/IJN.S30060
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author Hsieh, Dar-Shih
Lu, Hsiu-Chin
Chen, Cheng-Cheung
Wu, Chang-Jer
Yeh, Ming-Kung
author_facet Hsieh, Dar-Shih
Lu, Hsiu-Chin
Chen, Cheng-Cheung
Wu, Chang-Jer
Yeh, Ming-Kung
author_sort Hsieh, Dar-Shih
collection PubMed
description Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to −22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of −8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities.
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spelling pubmed-33570492012-05-21 The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system Hsieh, Dar-Shih Lu, Hsiu-Chin Chen, Cheng-Cheung Wu, Chang-Jer Yeh, Ming-Kung Int J Nanomedicine Original Research Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to −22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of −8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities. Dove Medical Press 2012 2012-03-28 /pmc/articles/PMC3357049/ /pubmed/22615529 http://dx.doi.org/10.2147/IJN.S30060 Text en © 2012 Hsieh et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Hsieh, Dar-Shih
Lu, Hsiu-Chin
Chen, Cheng-Cheung
Wu, Chang-Jer
Yeh, Ming-Kung
The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_full The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_fullStr The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_full_unstemmed The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_short The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_sort preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357049/
https://www.ncbi.nlm.nih.gov/pubmed/22615529
http://dx.doi.org/10.2147/IJN.S30060
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