Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells

We compared bona-fide human induced pluripotent stem cells (iPSC) derived from umbilical cord blood (CB) and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSC and K-iPSC are distinct in genome-wide DNA met...

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Autores principales: Kim, Kitai, Zhao, Rui, Doi, Akiko, Ng, Kitwa, Unternaehrer, Juli, Cahan, Patrick, Hongguang, Huo, Loh, Yuin-Han, Aryee, Martin J., Lensch, M. William, Li, Hu, Collins, James J., Feinberg, Andrew P., Daley, George Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357310/
https://www.ncbi.nlm.nih.gov/pubmed/22119740
http://dx.doi.org/10.1038/nbt.2052
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author Kim, Kitai
Zhao, Rui
Doi, Akiko
Ng, Kitwa
Unternaehrer, Juli
Cahan, Patrick
Hongguang, Huo
Loh, Yuin-Han
Aryee, Martin J.
Lensch, M. William
Li, Hu
Collins, James J.
Feinberg, Andrew P.
Daley, George Q.
author_facet Kim, Kitai
Zhao, Rui
Doi, Akiko
Ng, Kitwa
Unternaehrer, Juli
Cahan, Patrick
Hongguang, Huo
Loh, Yuin-Han
Aryee, Martin J.
Lensch, M. William
Li, Hu
Collins, James J.
Feinberg, Andrew P.
Daley, George Q.
author_sort Kim, Kitai
collection PubMed
description We compared bona-fide human induced pluripotent stem cells (iPSC) derived from umbilical cord blood (CB) and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSC and K-iPSC are distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture didn't improve their epigenetic resemblance to ESC, implying that some human iPSC retain a residual “epigenetic memory” of their tissue of origin.
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spelling pubmed-33573102012-06-01 Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells Kim, Kitai Zhao, Rui Doi, Akiko Ng, Kitwa Unternaehrer, Juli Cahan, Patrick Hongguang, Huo Loh, Yuin-Han Aryee, Martin J. Lensch, M. William Li, Hu Collins, James J. Feinberg, Andrew P. Daley, George Q. Nat Biotechnol Article We compared bona-fide human induced pluripotent stem cells (iPSC) derived from umbilical cord blood (CB) and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSC and K-iPSC are distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture didn't improve their epigenetic resemblance to ESC, implying that some human iPSC retain a residual “epigenetic memory” of their tissue of origin. 2011-11-27 /pmc/articles/PMC3357310/ /pubmed/22119740 http://dx.doi.org/10.1038/nbt.2052 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kim, Kitai
Zhao, Rui
Doi, Akiko
Ng, Kitwa
Unternaehrer, Juli
Cahan, Patrick
Hongguang, Huo
Loh, Yuin-Han
Aryee, Martin J.
Lensch, M. William
Li, Hu
Collins, James J.
Feinberg, Andrew P.
Daley, George Q.
Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title_full Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title_fullStr Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title_full_unstemmed Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title_short Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
title_sort donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357310/
https://www.ncbi.nlm.nih.gov/pubmed/22119740
http://dx.doi.org/10.1038/nbt.2052
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