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Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors

PURPOSE: Aggressive intervention against the bladder wall during transurethral resection of bladder tumors (TURBT) causes damage and leakage from blood vessels to the bladder lumen. The aim of this study was to determine whether TURBT could increase the level of circulating urothelial cells. METHODS...

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Autores principales: Antoniewicz, Artur A., Paziewska, Agnieszka, Mikula, Michal, Goryca, Krzysztof, Dabrowska, Michalina, Poletajew, Slawomir, Borowka, Andrzej, Ostrowski, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3358538/
https://www.ncbi.nlm.nih.gov/pubmed/22160796
http://dx.doi.org/10.1007/s11255-011-0102-z
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author Antoniewicz, Artur A.
Paziewska, Agnieszka
Mikula, Michal
Goryca, Krzysztof
Dabrowska, Michalina
Poletajew, Slawomir
Borowka, Andrzej
Ostrowski, Jerzy
author_facet Antoniewicz, Artur A.
Paziewska, Agnieszka
Mikula, Michal
Goryca, Krzysztof
Dabrowska, Michalina
Poletajew, Slawomir
Borowka, Andrzej
Ostrowski, Jerzy
author_sort Antoniewicz, Artur A.
collection PubMed
description PURPOSE: Aggressive intervention against the bladder wall during transurethral resection of bladder tumors (TURBT) causes damage and leakage from blood vessels to the bladder lumen. The aim of this study was to determine whether TURBT could increase the level of circulating urothelial cells. METHODS: Expression of tumor markers, discriminative for nucleated blood cells and urothelium, was evaluated by quantitative (q) RT-PCR on RNA isolated from peripheral blood samples of 51 patients who underwent TURBT for ≥cT1c bladder tumors. RESULTS: Four of 14 studied genes, epidermal growth factor receptor (EGFR), Collagen α-1(I) chain, Mast/stem cell growth factor receptor (KIT) and CD47, exhibited significant differences in gene expression between controls and cancer patients. While TURBT did not significantly increase the number of PCR-positive results of any transcripts, positive RT-PCR detection for EGFR was significantly less frequent on day 30 compared to results obtained before surgery. CONCLUSIONS: Although the results of our study do not provide evidence for increased tumor cell release into the peripheral blood after TURBT, they seem to indicate that EGFR mRNA measurement in the blood may provide useful information for urologists. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11255-011-0102-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-33585382012-05-31 Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors Antoniewicz, Artur A. Paziewska, Agnieszka Mikula, Michal Goryca, Krzysztof Dabrowska, Michalina Poletajew, Slawomir Borowka, Andrzej Ostrowski, Jerzy Int Urol Nephrol Urology – Original Paper PURPOSE: Aggressive intervention against the bladder wall during transurethral resection of bladder tumors (TURBT) causes damage and leakage from blood vessels to the bladder lumen. The aim of this study was to determine whether TURBT could increase the level of circulating urothelial cells. METHODS: Expression of tumor markers, discriminative for nucleated blood cells and urothelium, was evaluated by quantitative (q) RT-PCR on RNA isolated from peripheral blood samples of 51 patients who underwent TURBT for ≥cT1c bladder tumors. RESULTS: Four of 14 studied genes, epidermal growth factor receptor (EGFR), Collagen α-1(I) chain, Mast/stem cell growth factor receptor (KIT) and CD47, exhibited significant differences in gene expression between controls and cancer patients. While TURBT did not significantly increase the number of PCR-positive results of any transcripts, positive RT-PCR detection for EGFR was significantly less frequent on day 30 compared to results obtained before surgery. CONCLUSIONS: Although the results of our study do not provide evidence for increased tumor cell release into the peripheral blood after TURBT, they seem to indicate that EGFR mRNA measurement in the blood may provide useful information for urologists. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11255-011-0102-z) contains supplementary material, which is available to authorized users. Springer Netherlands 2011-12-09 2012 /pmc/articles/PMC3358538/ /pubmed/22160796 http://dx.doi.org/10.1007/s11255-011-0102-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Urology – Original Paper
Antoniewicz, Artur A.
Paziewska, Agnieszka
Mikula, Michal
Goryca, Krzysztof
Dabrowska, Michalina
Poletajew, Slawomir
Borowka, Andrzej
Ostrowski, Jerzy
Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title_full Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title_fullStr Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title_full_unstemmed Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title_short Lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
title_sort lack of evidence for increased level of circulating urothelial cells in the peripheral blood after transurethral resection of bladder tumors
topic Urology – Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3358538/
https://www.ncbi.nlm.nih.gov/pubmed/22160796
http://dx.doi.org/10.1007/s11255-011-0102-z
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