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Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model

AIM: To investigate the therapeutic effects of fluorouracil (5-Fu) and octreotide (Oct) continuous regional arterial infusion (CRAI,) alone or in combination, was administered in a canine model of severe acute pancreatitis (SAP). MATERIALS AND METHODS: The animals were divided into five groups; grou...

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Autores principales: Zhou, Meng Tao, Chen, Bi Cheng, Sun, Hong Wei, Jin, Yue Peng, Yang, Fa Jing, Zhang, Xing, Andersson, Roland, Zhang, Qi Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360032/
https://www.ncbi.nlm.nih.gov/pubmed/22655040
http://dx.doi.org/10.1371/journal.pone.0037347
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author Zhou, Meng Tao
Chen, Bi Cheng
Sun, Hong Wei
Jin, Yue Peng
Yang, Fa Jing
Zhang, Xing
Andersson, Roland
Zhang, Qi Yu
author_facet Zhou, Meng Tao
Chen, Bi Cheng
Sun, Hong Wei
Jin, Yue Peng
Yang, Fa Jing
Zhang, Xing
Andersson, Roland
Zhang, Qi Yu
author_sort Zhou, Meng Tao
collection PubMed
description AIM: To investigate the therapeutic effects of fluorouracil (5-Fu) and octreotide (Oct) continuous regional arterial infusion (CRAI,) alone or in combination, was administered in a canine model of severe acute pancreatitis (SAP). MATERIALS AND METHODS: The animals were divided into five groups; group A (Sham), group B (SAP), group C (SAP and 5-Fu), group D (SAP and Oct), and group E (SAP and 5-Fu + Oct). Levels of amylase, α-tumor necrosis factor (TNF-α), blood urea nitrogen (BUN), creatinine, thromboxane B2 and 6-keto- prostaglandin F1α were measured both before and after the induction of SAP. Pathologic examination of the pancreas and kidneys was performed after termination of the study. RESULTS: Pathologic changes noted in the pancreas in SAP significantly improved following CRAI with either single or combined administration of 5-Fu and Oct, where combination therapy demonstrated the lowest injury score. All treatment groups had significantly lower levels of serum TNF-α and amylase activity (P<0.05), though only groups D and E had a lower BUN level as compared to group B. The plasma thromboxane B(2) level increased in SAP, but the ratio of thromboxane B(2)/6-keto- prostaglandin F(1α) decreased in the treatment groups, with the combination therapy (group E) demonstrating the lowest ratio as compared to the other 3 experimental groups (P<0.05). CONCLUSIONS: The findings in the present study demonstrate an attenuation of SAP in a canine model following CRAI administration with 5-Fu or Oct, alone or in combination.
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spelling pubmed-33600322012-05-31 Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model Zhou, Meng Tao Chen, Bi Cheng Sun, Hong Wei Jin, Yue Peng Yang, Fa Jing Zhang, Xing Andersson, Roland Zhang, Qi Yu PLoS One Research Article AIM: To investigate the therapeutic effects of fluorouracil (5-Fu) and octreotide (Oct) continuous regional arterial infusion (CRAI,) alone or in combination, was administered in a canine model of severe acute pancreatitis (SAP). MATERIALS AND METHODS: The animals were divided into five groups; group A (Sham), group B (SAP), group C (SAP and 5-Fu), group D (SAP and Oct), and group E (SAP and 5-Fu + Oct). Levels of amylase, α-tumor necrosis factor (TNF-α), blood urea nitrogen (BUN), creatinine, thromboxane B2 and 6-keto- prostaglandin F1α were measured both before and after the induction of SAP. Pathologic examination of the pancreas and kidneys was performed after termination of the study. RESULTS: Pathologic changes noted in the pancreas in SAP significantly improved following CRAI with either single or combined administration of 5-Fu and Oct, where combination therapy demonstrated the lowest injury score. All treatment groups had significantly lower levels of serum TNF-α and amylase activity (P<0.05), though only groups D and E had a lower BUN level as compared to group B. The plasma thromboxane B(2) level increased in SAP, but the ratio of thromboxane B(2)/6-keto- prostaglandin F(1α) decreased in the treatment groups, with the combination therapy (group E) demonstrating the lowest ratio as compared to the other 3 experimental groups (P<0.05). CONCLUSIONS: The findings in the present study demonstrate an attenuation of SAP in a canine model following CRAI administration with 5-Fu or Oct, alone or in combination. Public Library of Science 2012-05-24 /pmc/articles/PMC3360032/ /pubmed/22655040 http://dx.doi.org/10.1371/journal.pone.0037347 Text en Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhou, Meng Tao
Chen, Bi Cheng
Sun, Hong Wei
Jin, Yue Peng
Yang, Fa Jing
Zhang, Xing
Andersson, Roland
Zhang, Qi Yu
Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title_full Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title_fullStr Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title_full_unstemmed Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title_short Continuous Regional Arterial Infusion with Fluorouracil and Octreotide Attenuates Severe Acute Pancreatitis in a Canine Model
title_sort continuous regional arterial infusion with fluorouracil and octreotide attenuates severe acute pancreatitis in a canine model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360032/
https://www.ncbi.nlm.nih.gov/pubmed/22655040
http://dx.doi.org/10.1371/journal.pone.0037347
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