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Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco

BACKGROUND: Dufulin is a new antiviral agent that is highly effective against plant viruses and acts by activating systemic acquired resistance (SAR) in plants. In recent years, it has been used widely to prevent and control tobacco and rice viral diseases in China. However, its targets and mechanis...

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Autores principales: Chen, Zhuo, Zeng, Mengjiao, Song, Baoan, Hou, Chengrui, Hu, Deyu, Li, Xiangyang, Wang, Zhenchao, Fan, Huitao, Bi, Liang, Liu, Jiaju, Yu, Dandan, Jin, Linhong, Yang, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360678/
https://www.ncbi.nlm.nih.gov/pubmed/22662252
http://dx.doi.org/10.1371/journal.pone.0037944
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author Chen, Zhuo
Zeng, Mengjiao
Song, Baoan
Hou, Chengrui
Hu, Deyu
Li, Xiangyang
Wang, Zhenchao
Fan, Huitao
Bi, Liang
Liu, Jiaju
Yu, Dandan
Jin, Linhong
Yang, Song
author_facet Chen, Zhuo
Zeng, Mengjiao
Song, Baoan
Hou, Chengrui
Hu, Deyu
Li, Xiangyang
Wang, Zhenchao
Fan, Huitao
Bi, Liang
Liu, Jiaju
Yu, Dandan
Jin, Linhong
Yang, Song
author_sort Chen, Zhuo
collection PubMed
description BACKGROUND: Dufulin is a new antiviral agent that is highly effective against plant viruses and acts by activating systemic acquired resistance (SAR) in plants. In recent years, it has been used widely to prevent and control tobacco and rice viral diseases in China. However, its targets and mechanism of action are still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Here, differential in-gel electrophoresis (DIGE) and classical two-dimensional electrophoresis (2-DE) techniques were combined with mass spectrometry (MS) to identify the target of Dufulin. More than 40 proteins were found to be differentially expressed (≥1.5 fold or ≤1.5 fold) upon Dufulin treatment in Nicotiana tabacum K(326). Based on annotations in the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, these proteins were found to be related to disease resistance. Directed acyclic graph (DAG) analysis of the various pathways demonstrated harpin binding protein-1 (HrBP1) as the target of action of Dufulin. Additionally, western blotting, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and real time PCR analyses were also conducted to identify the specific mechanism of action of Dufulin. Our results show that activation of HrBP1 triggers the salicylic acid (SA) signaling pathway and thereby produces antiviral responses in the plant host. A protective assay based on lesion counting further confirmed the antiviral activity of Dufulin. CONCLUSION: This study identified HrBP1 as a target protein of Dufulin and that Dufulin can activate the SA signaling pathway to induce host plants to generate antiviral responses.
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spelling pubmed-33606782012-06-01 Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco Chen, Zhuo Zeng, Mengjiao Song, Baoan Hou, Chengrui Hu, Deyu Li, Xiangyang Wang, Zhenchao Fan, Huitao Bi, Liang Liu, Jiaju Yu, Dandan Jin, Linhong Yang, Song PLoS One Research Article BACKGROUND: Dufulin is a new antiviral agent that is highly effective against plant viruses and acts by activating systemic acquired resistance (SAR) in plants. In recent years, it has been used widely to prevent and control tobacco and rice viral diseases in China. However, its targets and mechanism of action are still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Here, differential in-gel electrophoresis (DIGE) and classical two-dimensional electrophoresis (2-DE) techniques were combined with mass spectrometry (MS) to identify the target of Dufulin. More than 40 proteins were found to be differentially expressed (≥1.5 fold or ≤1.5 fold) upon Dufulin treatment in Nicotiana tabacum K(326). Based on annotations in the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, these proteins were found to be related to disease resistance. Directed acyclic graph (DAG) analysis of the various pathways demonstrated harpin binding protein-1 (HrBP1) as the target of action of Dufulin. Additionally, western blotting, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and real time PCR analyses were also conducted to identify the specific mechanism of action of Dufulin. Our results show that activation of HrBP1 triggers the salicylic acid (SA) signaling pathway and thereby produces antiviral responses in the plant host. A protective assay based on lesion counting further confirmed the antiviral activity of Dufulin. CONCLUSION: This study identified HrBP1 as a target protein of Dufulin and that Dufulin can activate the SA signaling pathway to induce host plants to generate antiviral responses. Public Library of Science 2012-05-25 /pmc/articles/PMC3360678/ /pubmed/22662252 http://dx.doi.org/10.1371/journal.pone.0037944 Text en Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Zhuo
Zeng, Mengjiao
Song, Baoan
Hou, Chengrui
Hu, Deyu
Li, Xiangyang
Wang, Zhenchao
Fan, Huitao
Bi, Liang
Liu, Jiaju
Yu, Dandan
Jin, Linhong
Yang, Song
Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title_full Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title_fullStr Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title_full_unstemmed Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title_short Dufulin Activates HrBP1 to Produce Antiviral Responses in Tobacco
title_sort dufulin activates hrbp1 to produce antiviral responses in tobacco
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360678/
https://www.ncbi.nlm.nih.gov/pubmed/22662252
http://dx.doi.org/10.1371/journal.pone.0037944
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