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Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings

A biofouling simulation system consisting of a flow cell and a recirculation tank was used. The fluid circulates at a flow rate of 350 L· h(−1) in a semicircular flow cell with hydraulic diameter of 18.3 mm, corresponding to an average velocity of 0.275 m· s(−1). Using computational fluid dynamics f...

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Detalles Bibliográficos
Autores principales: Teodósio, Joana S., Simões, Manuel, Alves, Manuel A., Melo, Luís F., Mergulhão, Filipe J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361220/
https://www.ncbi.nlm.nih.gov/pubmed/22666110
http://dx.doi.org/10.1100/2012/361496
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author Teodósio, Joana S.
Simões, Manuel
Alves, Manuel A.
Melo, Luís F.
Mergulhão, Filipe J.
author_facet Teodósio, Joana S.
Simões, Manuel
Alves, Manuel A.
Melo, Luís F.
Mergulhão, Filipe J.
author_sort Teodósio, Joana S.
collection PubMed
description A biofouling simulation system consisting of a flow cell and a recirculation tank was used. The fluid circulates at a flow rate of 350 L· h(−1) in a semicircular flow cell with hydraulic diameter of 18.3 mm, corresponding to an average velocity of 0.275 m· s(−1). Using computational fluid dynamics for flow simulation, an average wall shear stress of 0.4 Pa was predicted. The validity of the numerical simulations was visually confirmed by inorganic deposit formation (using kaolin particles) and also by direct observation of pathlines of tracer PVC particles using streak photography. Furthermore, the validity of chemostat assumptions was verified by residence time analysis. The system was used to assess the influence of the dilution rate on biofilm formation by Escherichia coli JM109(DE3). Two dilution rates of 0.013 and 0.0043 h(−1) were tested and the results show that the planktonic cell concentration is increased at the lower dilution rate and that no significant changes were detected on the amount of biofilm formed in both conditions.
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spelling pubmed-33612202012-06-04 Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings Teodósio, Joana S. Simões, Manuel Alves, Manuel A. Melo, Luís F. Mergulhão, Filipe J. ScientificWorldJournal Research Article A biofouling simulation system consisting of a flow cell and a recirculation tank was used. The fluid circulates at a flow rate of 350 L· h(−1) in a semicircular flow cell with hydraulic diameter of 18.3 mm, corresponding to an average velocity of 0.275 m· s(−1). Using computational fluid dynamics for flow simulation, an average wall shear stress of 0.4 Pa was predicted. The validity of the numerical simulations was visually confirmed by inorganic deposit formation (using kaolin particles) and also by direct observation of pathlines of tracer PVC particles using streak photography. Furthermore, the validity of chemostat assumptions was verified by residence time analysis. The system was used to assess the influence of the dilution rate on biofilm formation by Escherichia coli JM109(DE3). Two dilution rates of 0.013 and 0.0043 h(−1) were tested and the results show that the planktonic cell concentration is increased at the lower dilution rate and that no significant changes were detected on the amount of biofilm formed in both conditions. The Scientific World Journal 2012-04-26 /pmc/articles/PMC3361220/ /pubmed/22666110 http://dx.doi.org/10.1100/2012/361496 Text en Copyright © 2012 Joana S. Teodósio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Teodósio, Joana S.
Simões, Manuel
Alves, Manuel A.
Melo, Luís F.
Mergulhão, Filipe J.
Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title_full Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title_fullStr Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title_full_unstemmed Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title_short Setup and Validation of Flow Cell Systems for Biofouling Simulation in Industrial Settings
title_sort setup and validation of flow cell systems for biofouling simulation in industrial settings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361220/
https://www.ncbi.nlm.nih.gov/pubmed/22666110
http://dx.doi.org/10.1100/2012/361496
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