Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis

In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains...

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Autores principales: Rouabhia, Mahmoud, Semlali, Abdelhabib, Chandra, Jyotsna, Mukherjee, Pranab, Chmielewski, Witold, Ghannoum, Mahmoud A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361342/
https://www.ncbi.nlm.nih.gov/pubmed/22665950
http://dx.doi.org/10.1155/2012/398207
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author Rouabhia, Mahmoud
Semlali, Abdelhabib
Chandra, Jyotsna
Mukherjee, Pranab
Chmielewski, Witold
Ghannoum, Mahmoud A.
author_facet Rouabhia, Mahmoud
Semlali, Abdelhabib
Chandra, Jyotsna
Mukherjee, Pranab
Chmielewski, Witold
Ghannoum, Mahmoud A.
author_sort Rouabhia, Mahmoud
collection PubMed
description In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen). We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida.
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spelling pubmed-33613422012-06-04 Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis Rouabhia, Mahmoud Semlali, Abdelhabib Chandra, Jyotsna Mukherjee, Pranab Chmielewski, Witold Ghannoum, Mahmoud A. Mediators Inflamm Research Article In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen). We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida. Hindawi Publishing Corporation 2012 2012-05-14 /pmc/articles/PMC3361342/ /pubmed/22665950 http://dx.doi.org/10.1155/2012/398207 Text en Copyright © 2012 Mahmoud Rouabhia et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rouabhia, Mahmoud
Semlali, Abdelhabib
Chandra, Jyotsna
Mukherjee, Pranab
Chmielewski, Witold
Ghannoum, Mahmoud A.
Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title_full Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title_fullStr Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title_full_unstemmed Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title_short Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
title_sort disruption of the ecm33 gene in candida albicans prevents biofilm formation, engineered human oral mucosa tissue damage and gingival cell necrosis/apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361342/
https://www.ncbi.nlm.nih.gov/pubmed/22665950
http://dx.doi.org/10.1155/2012/398207
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