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Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma
PURPOSE: To study ultrastructural changes of the trabecular meshwork in acute and chronic primary angle closure glaucoma (PACG) and primary open angle glaucoma (POAG) eyes by scanning electron microscopy. MATERIALS AND METHODS: Twenty-one trabecular meshwork surgical specimens from consecutive glauc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361812/ https://www.ncbi.nlm.nih.gov/pubmed/22569378 http://dx.doi.org/10.4103/0301-4738.95868 |
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author | Sihota, Ramanjit Goyal, Amita Kaur, Jasbir Gupta, Viney Nag, Tapas C |
author_facet | Sihota, Ramanjit Goyal, Amita Kaur, Jasbir Gupta, Viney Nag, Tapas C |
author_sort | Sihota, Ramanjit |
collection | PubMed |
description | PURPOSE: To study ultrastructural changes of the trabecular meshwork in acute and chronic primary angle closure glaucoma (PACG) and primary open angle glaucoma (POAG) eyes by scanning electron microscopy. MATERIALS AND METHODS: Twenty-one trabecular meshwork surgical specimens from consecutive glaucomatous eyes after a trabeculectomy and five postmortem corneoscleral specimens were fixed immediately in Karnovsky solution. The tissues were washed in 0.1 M phosphate buffer saline, post-fixed in 1% osmium tetraoxide, dehydrated in acetone series (30-100%), dried and mounted. RESULTS: Normal trabecular tissue showed well-defined, thin, cylindrical uveal trabecular beams with many large spaces, overlying flatter corneoscleral beams and numerous smaller spaces. In acute PACG eyes, the trabecular meshwork showed grossly swollen, irregular trabecular endothelial cells with intercellular and occasional basal separation with few spaces. Numerous activated macrophages, leucocytes and amorphous debris were present. Chronic PACG eyes had a few, thickened posterior uveal trabecular beams visible. A homogenous deposit covered the anterior uveal trabeculae and spaces. Converging, fan-shaped trabecular beam configuration corresponded to gonioscopic areas of peripheral anterior synechiae. In POAG eyes, anterior uveal trabecular beams were thin and strap-like, while those posteriorly were wide, with a homogenous deposit covering and bridging intertrabecular spaces, especially posteriorly. Underlying corneoscleral trabecular layers and spaces were visualized in some areas. CONCLUSIONS: In acute PACG a marked edema of the endothelium probably contributes for the acute and marked intraocular pressure (IOP) elevation. Chronically raised IOP in chronic PACG and POAG probably results, at least in part, from decreased aqueous outflow secondary to widening and fusion of adjacent trabecular beams, together with the homogenous deposit enmeshing trabecular beams and spaces. |
format | Online Article Text |
id | pubmed-3361812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-33618122012-06-01 Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma Sihota, Ramanjit Goyal, Amita Kaur, Jasbir Gupta, Viney Nag, Tapas C Indian J Ophthalmol Original Article PURPOSE: To study ultrastructural changes of the trabecular meshwork in acute and chronic primary angle closure glaucoma (PACG) and primary open angle glaucoma (POAG) eyes by scanning electron microscopy. MATERIALS AND METHODS: Twenty-one trabecular meshwork surgical specimens from consecutive glaucomatous eyes after a trabeculectomy and five postmortem corneoscleral specimens were fixed immediately in Karnovsky solution. The tissues were washed in 0.1 M phosphate buffer saline, post-fixed in 1% osmium tetraoxide, dehydrated in acetone series (30-100%), dried and mounted. RESULTS: Normal trabecular tissue showed well-defined, thin, cylindrical uveal trabecular beams with many large spaces, overlying flatter corneoscleral beams and numerous smaller spaces. In acute PACG eyes, the trabecular meshwork showed grossly swollen, irregular trabecular endothelial cells with intercellular and occasional basal separation with few spaces. Numerous activated macrophages, leucocytes and amorphous debris were present. Chronic PACG eyes had a few, thickened posterior uveal trabecular beams visible. A homogenous deposit covered the anterior uveal trabeculae and spaces. Converging, fan-shaped trabecular beam configuration corresponded to gonioscopic areas of peripheral anterior synechiae. In POAG eyes, anterior uveal trabecular beams were thin and strap-like, while those posteriorly were wide, with a homogenous deposit covering and bridging intertrabecular spaces, especially posteriorly. Underlying corneoscleral trabecular layers and spaces were visualized in some areas. CONCLUSIONS: In acute PACG a marked edema of the endothelium probably contributes for the acute and marked intraocular pressure (IOP) elevation. Chronically raised IOP in chronic PACG and POAG probably results, at least in part, from decreased aqueous outflow secondary to widening and fusion of adjacent trabecular beams, together with the homogenous deposit enmeshing trabecular beams and spaces. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3361812/ /pubmed/22569378 http://dx.doi.org/10.4103/0301-4738.95868 Text en Copyright: © Indian Journal of Ophthalmology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sihota, Ramanjit Goyal, Amita Kaur, Jasbir Gupta, Viney Nag, Tapas C Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title | Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title_full | Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title_fullStr | Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title_full_unstemmed | Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title_short | Scanning electron microscopy of the trabecular meshwork: Understanding the pathogenesis of primary angle closure glaucoma |
title_sort | scanning electron microscopy of the trabecular meshwork: understanding the pathogenesis of primary angle closure glaucoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361812/ https://www.ncbi.nlm.nih.gov/pubmed/22569378 http://dx.doi.org/10.4103/0301-4738.95868 |
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