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Imunocompetent Mice Model for Dengue Virus Infection

Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased stead...

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Autores principales: Gonçalves, Denise, de Queiroz Prado, Rafael, Almeida Xavier, Eric, Cristina de Oliveira, Natália, da Matta Guedes, Paulo Marcos, da Silva, João Santana, Moraes Figueiredo, Luiz Tadeu, Aquino, Victor Hugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362018/
https://www.ncbi.nlm.nih.gov/pubmed/22666132
http://dx.doi.org/10.1100/2012/525947
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author Gonçalves, Denise
de Queiroz Prado, Rafael
Almeida Xavier, Eric
Cristina de Oliveira, Natália
da Matta Guedes, Paulo Marcos
da Silva, João Santana
Moraes Figueiredo, Luiz Tadeu
Aquino, Victor Hugo
author_facet Gonçalves, Denise
de Queiroz Prado, Rafael
Almeida Xavier, Eric
Cristina de Oliveira, Natália
da Matta Guedes, Paulo Marcos
da Silva, João Santana
Moraes Figueiredo, Luiz Tadeu
Aquino, Victor Hugo
author_sort Gonçalves, Denise
collection PubMed
description Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.
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spelling pubmed-33620182012-06-04 Imunocompetent Mice Model for Dengue Virus Infection Gonçalves, Denise de Queiroz Prado, Rafael Almeida Xavier, Eric Cristina de Oliveira, Natália da Matta Guedes, Paulo Marcos da Silva, João Santana Moraes Figueiredo, Luiz Tadeu Aquino, Victor Hugo ScientificWorldJournal Research Article Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines. The Scientific World Journal 2012-05-15 /pmc/articles/PMC3362018/ /pubmed/22666132 http://dx.doi.org/10.1100/2012/525947 Text en Copyright © 2012 Denise Gonçalves et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gonçalves, Denise
de Queiroz Prado, Rafael
Almeida Xavier, Eric
Cristina de Oliveira, Natália
da Matta Guedes, Paulo Marcos
da Silva, João Santana
Moraes Figueiredo, Luiz Tadeu
Aquino, Victor Hugo
Imunocompetent Mice Model for Dengue Virus Infection
title Imunocompetent Mice Model for Dengue Virus Infection
title_full Imunocompetent Mice Model for Dengue Virus Infection
title_fullStr Imunocompetent Mice Model for Dengue Virus Infection
title_full_unstemmed Imunocompetent Mice Model for Dengue Virus Infection
title_short Imunocompetent Mice Model for Dengue Virus Infection
title_sort imunocompetent mice model for dengue virus infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362018/
https://www.ncbi.nlm.nih.gov/pubmed/22666132
http://dx.doi.org/10.1100/2012/525947
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