Cargando…

Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin

A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequence...

Descripción completa

Detalles Bibliográficos
Autores principales: Dallo, Shatha F., Zhang, Bailin, Denno, James, Hong, Soonbae, Tsai, Anyu, Haskins, Williams, Ye, Jing Yong, Weitao, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362023/
https://www.ncbi.nlm.nih.gov/pubmed/22666090
http://dx.doi.org/10.1100/2012/128705
_version_ 1782234188241436672
author Dallo, Shatha F.
Zhang, Bailin
Denno, James
Hong, Soonbae
Tsai, Anyu
Haskins, Williams
Ye, Jing Yong
Weitao, Tao
author_facet Dallo, Shatha F.
Zhang, Bailin
Denno, James
Hong, Soonbae
Tsai, Anyu
Haskins, Williams
Ye, Jing Yong
Weitao, Tao
author_sort Dallo, Shatha F.
collection PubMed
description A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs) was revealed by immune electron microscopy, and its presence in the outer membrane (OM) and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR) configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin.
format Online
Article
Text
id pubmed-3362023
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Scientific World Journal
record_format MEDLINE/PubMed
spelling pubmed-33620232012-06-04 Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin Dallo, Shatha F. Zhang, Bailin Denno, James Hong, Soonbae Tsai, Anyu Haskins, Williams Ye, Jing Yong Weitao, Tao ScientificWorldJournal Research Article A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs) was revealed by immune electron microscopy, and its presence in the outer membrane (OM) and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR) configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin. The Scientific World Journal 2012-05-15 /pmc/articles/PMC3362023/ /pubmed/22666090 http://dx.doi.org/10.1100/2012/128705 Text en Copyright © 2012 Shatha F. Dallo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dallo, Shatha F.
Zhang, Bailin
Denno, James
Hong, Soonbae
Tsai, Anyu
Haskins, Williams
Ye, Jing Yong
Weitao, Tao
Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title_full Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title_fullStr Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title_full_unstemmed Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title_short Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
title_sort association of acinetobacter baumannii ef-tu with cell surface, outer membrane vesicles, and fibronectin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362023/
https://www.ncbi.nlm.nih.gov/pubmed/22666090
http://dx.doi.org/10.1100/2012/128705
work_keys_str_mv AT dalloshathaf associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT zhangbailin associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT dennojames associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT hongsoonbae associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT tsaianyu associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT haskinswilliams associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT yejingyong associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin
AT weitaotao associationofacinetobacterbaumanniieftuwithcellsurfaceoutermembranevesiclesandfibronectin