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Two Chromatographic Methods for the Determination of Some Antimigraine Drugs
Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: met...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362328/ https://www.ncbi.nlm.nih.gov/pubmed/22654488 http://dx.doi.org/10.4137/ACI.S8864 |
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author | El-Bagary, Ramzia I. Mohammed, Nashwah G. Nasr, Heba A. |
author_facet | El-Bagary, Ramzia I. Mohammed, Nashwah G. Nasr, Heba A. |
author_sort | El-Bagary, Ramzia I. |
collection | PubMed |
description | Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: methanol: dichloromethane: ammonia (10:6:3:1 v/v), ethyl acetate: methanol: ammonia (15:4:1 v/v), and methanol: acetonitrile: ammonia (9:4:1 v/v) for almotriptan, eletriptan and rizatriptan respectively. The TLC plates were scanned at 235 nm. Linear relationships were obtained over concentration ranges (5–50 μg/spot) for almotriptan and rizatriptan, and (5–60 μg/spot) for eletriptan. The second method based on the separation and determination of the studied drugs, using RP-HPLC technique. The separation was achieved on C18 Hypersil column, elution was carried out using phosphate buffer pH 3: methanol: acetonitrile (2: 1:1 v/v) at flow rate 2 mL/min and UV detection at 235 nm. Linear relationships were obtained over concentration ranges (10–200 μg/mL) for almotriptan and eletriptan, and (10–180 μg/mL) for rizatriptan. The chromatographic methods were successfully applied for the determination of each of the studied drugs in pure form, tablet form, and in laboratory prepared mixtures with their acid degradation products. |
format | Online Article Text |
id | pubmed-3362328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-33623282012-05-31 Two Chromatographic Methods for the Determination of Some Antimigraine Drugs El-Bagary, Ramzia I. Mohammed, Nashwah G. Nasr, Heba A. Anal Chem Insights Original Research Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: methanol: dichloromethane: ammonia (10:6:3:1 v/v), ethyl acetate: methanol: ammonia (15:4:1 v/v), and methanol: acetonitrile: ammonia (9:4:1 v/v) for almotriptan, eletriptan and rizatriptan respectively. The TLC plates were scanned at 235 nm. Linear relationships were obtained over concentration ranges (5–50 μg/spot) for almotriptan and rizatriptan, and (5–60 μg/spot) for eletriptan. The second method based on the separation and determination of the studied drugs, using RP-HPLC technique. The separation was achieved on C18 Hypersil column, elution was carried out using phosphate buffer pH 3: methanol: acetonitrile (2: 1:1 v/v) at flow rate 2 mL/min and UV detection at 235 nm. Linear relationships were obtained over concentration ranges (10–200 μg/mL) for almotriptan and eletriptan, and (10–180 μg/mL) for rizatriptan. The chromatographic methods were successfully applied for the determination of each of the studied drugs in pure form, tablet form, and in laboratory prepared mixtures with their acid degradation products. Libertas Academica 2012-05-14 /pmc/articles/PMC3362328/ /pubmed/22654488 http://dx.doi.org/10.4137/ACI.S8864 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |
spellingShingle | Original Research El-Bagary, Ramzia I. Mohammed, Nashwah G. Nasr, Heba A. Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title | Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title_full | Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title_fullStr | Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title_full_unstemmed | Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title_short | Two Chromatographic Methods for the Determination of Some Antimigraine Drugs |
title_sort | two chromatographic methods for the determination of some antimigraine drugs |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362328/ https://www.ncbi.nlm.nih.gov/pubmed/22654488 http://dx.doi.org/10.4137/ACI.S8864 |
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