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High-Throughput Sequencing of 16S rRNA Gene Amplicons: Effects of Extraction Procedure, Primer Length and Annealing Temperature

The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing. Although considerable effort has been devoted to identifying the most informative region of the 16S gene and the o...

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Detalles Bibliográficos
Autores principales:	Sergeant, Martin J., Constantinidou, Chrystala, Cogan, Tristan, Penn, Charles W., Pallen, Mark J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362549/ https://www.ncbi.nlm.nih.gov/pubmed/22666455 http://dx.doi.org/10.1371/journal.pone.0038094

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