Cargando…
Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles
Bank voles free living in a contaminated environment have been shown to be more sensitive to cadmium (Cd) toxicity than the rodents exposed to Cd under laboratory conditions. The objective of this study was to find out whether benzo(a)pyrene (BaP), a common environmental co-contaminant, increases Cd...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Humana Press Inc
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362696/ https://www.ncbi.nlm.nih.gov/pubmed/22124863 http://dx.doi.org/10.1007/s12011-011-9279-3 |
_version_ | 1782234240870514688 |
---|---|
author | Salińska, Aneta Włostowski, Tadeusz Maciak, Sebastian Łaszkiewicz-Tiszczenko, Barbara Kozłowski, Paweł |
author_facet | Salińska, Aneta Włostowski, Tadeusz Maciak, Sebastian Łaszkiewicz-Tiszczenko, Barbara Kozłowski, Paweł |
author_sort | Salińska, Aneta |
collection | PubMed |
description | Bank voles free living in a contaminated environment have been shown to be more sensitive to cadmium (Cd) toxicity than the rodents exposed to Cd under laboratory conditions. The objective of this study was to find out whether benzo(a)pyrene (BaP), a common environmental co-contaminant, increases Cd toxicity through inhibition of metallothionein (MT) synthesis—a low molecular weight protein that is considered to be primary intracellular component of the protective mechanism. For 6 weeks, the female bank voles were provided with diet containing Cd [less than 0.1 μg/g (control) and 60 μg/g dry wt.] and BaP (0, 5, and 10 μg/g dry wt.) alone or in combination. At the end of exposure period, apoptosis and analyses of MT, Cd, and zinc (Zn) in the liver and kidneys were carried out. Dietary BaP 5 μg/g did not affect but BaP 10 μg/g potentiated rather than inhibited induction of hepatic and renal MT by Cd, and diminished Cd-induced apoptosis in both organs. The hepatic and renal Zn followed a pattern similar to that of MT, attaining the highest level in the Cd + BaP 10-μg/g group. These data indicate that dietary BaP attenuates rather than exacerbates Cd toxicity in bank voles, probably by potentiating MT synthesis and increasing Zn concentration in the liver and kidneys. |
format | Online Article Text |
id | pubmed-3362696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Humana Press Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-33626962012-06-13 Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles Salińska, Aneta Włostowski, Tadeusz Maciak, Sebastian Łaszkiewicz-Tiszczenko, Barbara Kozłowski, Paweł Biol Trace Elem Res Article Bank voles free living in a contaminated environment have been shown to be more sensitive to cadmium (Cd) toxicity than the rodents exposed to Cd under laboratory conditions. The objective of this study was to find out whether benzo(a)pyrene (BaP), a common environmental co-contaminant, increases Cd toxicity through inhibition of metallothionein (MT) synthesis—a low molecular weight protein that is considered to be primary intracellular component of the protective mechanism. For 6 weeks, the female bank voles were provided with diet containing Cd [less than 0.1 μg/g (control) and 60 μg/g dry wt.] and BaP (0, 5, and 10 μg/g dry wt.) alone or in combination. At the end of exposure period, apoptosis and analyses of MT, Cd, and zinc (Zn) in the liver and kidneys were carried out. Dietary BaP 5 μg/g did not affect but BaP 10 μg/g potentiated rather than inhibited induction of hepatic and renal MT by Cd, and diminished Cd-induced apoptosis in both organs. The hepatic and renal Zn followed a pattern similar to that of MT, attaining the highest level in the Cd + BaP 10-μg/g group. These data indicate that dietary BaP attenuates rather than exacerbates Cd toxicity in bank voles, probably by potentiating MT synthesis and increasing Zn concentration in the liver and kidneys. Humana Press Inc 2011-11-29 2012 /pmc/articles/PMC3362696/ /pubmed/22124863 http://dx.doi.org/10.1007/s12011-011-9279-3 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Salińska, Aneta Włostowski, Tadeusz Maciak, Sebastian Łaszkiewicz-Tiszczenko, Barbara Kozłowski, Paweł Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title | Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title_full | Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title_fullStr | Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title_full_unstemmed | Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title_short | Combined Effect of Dietary Cadmium and Benzo(a)pyrene on Metallothionein Induction and Apoptosis in the Liver and Kidneys of Bank Voles |
title_sort | combined effect of dietary cadmium and benzo(a)pyrene on metallothionein induction and apoptosis in the liver and kidneys of bank voles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362696/ https://www.ncbi.nlm.nih.gov/pubmed/22124863 http://dx.doi.org/10.1007/s12011-011-9279-3 |
work_keys_str_mv | AT salinskaaneta combinedeffectofdietarycadmiumandbenzoapyreneonmetallothioneininductionandapoptosisintheliverandkidneysofbankvoles AT włostowskitadeusz combinedeffectofdietarycadmiumandbenzoapyreneonmetallothioneininductionandapoptosisintheliverandkidneysofbankvoles AT maciaksebastian combinedeffectofdietarycadmiumandbenzoapyreneonmetallothioneininductionandapoptosisintheliverandkidneysofbankvoles AT łaszkiewicztiszczenkobarbara combinedeffectofdietarycadmiumandbenzoapyreneonmetallothioneininductionandapoptosisintheliverandkidneysofbankvoles AT kozłowskipaweł combinedeffectofdietarycadmiumandbenzoapyreneonmetallothioneininductionandapoptosisintheliverandkidneysofbankvoles |