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Regulation of Pancreatic microRNA-7 Expression
Genome-encoded microRNAs (miRNAs) provide a posttranscriptional regulatory layer, which is important for pancreas development. Differentiation of endocrine cells is controlled by a network of pancreatic transcription factors including Ngn3 and NeuroD/Beta2. However, how specific miRNAs are intertwin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362837/ https://www.ncbi.nlm.nih.gov/pubmed/22675342 http://dx.doi.org/10.1155/2012/695214 |
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author | Kredo-Russo, Sharon Ness, Avital Mandelbaum, Amitai D. Walker, Michael D. Hornstein, Eran |
author_facet | Kredo-Russo, Sharon Ness, Avital Mandelbaum, Amitai D. Walker, Michael D. Hornstein, Eran |
author_sort | Kredo-Russo, Sharon |
collection | PubMed |
description | Genome-encoded microRNAs (miRNAs) provide a posttranscriptional regulatory layer, which is important for pancreas development. Differentiation of endocrine cells is controlled by a network of pancreatic transcription factors including Ngn3 and NeuroD/Beta2. However, how specific miRNAs are intertwined into this transcriptional network is not well understood. Here, we characterize the regulation of microRNA-7 (miR-7) by endocrine-specific transcription factors. Our data reveal that three independent miR-7 genes are coexpressed in the pancreas. We have identified conserved blocks upstream of pre-miR-7a-2 and pre-miR-7b and demonstrated by functional assays that they possess promoter activity, which is increased by the expression of NeuroD/Beta2. These data suggest that the endocrine specificity of miR-7 expression is governed by transcriptional mechanisms and involves members of the pancreatic endocrine network of transcription factors. |
format | Online Article Text |
id | pubmed-3362837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33628372012-06-06 Regulation of Pancreatic microRNA-7 Expression Kredo-Russo, Sharon Ness, Avital Mandelbaum, Amitai D. Walker, Michael D. Hornstein, Eran Exp Diabetes Res Research Article Genome-encoded microRNAs (miRNAs) provide a posttranscriptional regulatory layer, which is important for pancreas development. Differentiation of endocrine cells is controlled by a network of pancreatic transcription factors including Ngn3 and NeuroD/Beta2. However, how specific miRNAs are intertwined into this transcriptional network is not well understood. Here, we characterize the regulation of microRNA-7 (miR-7) by endocrine-specific transcription factors. Our data reveal that three independent miR-7 genes are coexpressed in the pancreas. We have identified conserved blocks upstream of pre-miR-7a-2 and pre-miR-7b and demonstrated by functional assays that they possess promoter activity, which is increased by the expression of NeuroD/Beta2. These data suggest that the endocrine specificity of miR-7 expression is governed by transcriptional mechanisms and involves members of the pancreatic endocrine network of transcription factors. Hindawi Publishing Corporation 2012 2012-05-17 /pmc/articles/PMC3362837/ /pubmed/22675342 http://dx.doi.org/10.1155/2012/695214 Text en Copyright © 2012 Sharon Kredo-Russo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kredo-Russo, Sharon Ness, Avital Mandelbaum, Amitai D. Walker, Michael D. Hornstein, Eran Regulation of Pancreatic microRNA-7 Expression |
title | Regulation of Pancreatic microRNA-7 Expression |
title_full | Regulation of Pancreatic microRNA-7 Expression |
title_fullStr | Regulation of Pancreatic microRNA-7 Expression |
title_full_unstemmed | Regulation of Pancreatic microRNA-7 Expression |
title_short | Regulation of Pancreatic microRNA-7 Expression |
title_sort | regulation of pancreatic microrna-7 expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362837/ https://www.ncbi.nlm.nih.gov/pubmed/22675342 http://dx.doi.org/10.1155/2012/695214 |
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