Cargando…
Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis?
Previous studies showed that several members of the S100A family are involved in neovascularization and tumor development. This study checked whether low concentrations of S100A8 or S100A9 has any effect on the behaviour of vascular endothelial cells. A human umbilical vascular endothelial cell (HUV...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3363068/ https://www.ncbi.nlm.nih.gov/pubmed/22685372 http://dx.doi.org/10.1155/2012/248574 |
_version_ | 1782234295464624128 |
---|---|
author | Li, Changyou Li, Siyuan Jia, Changkai Yang, Lingling Song, Zicheng Wang, Yiqiang |
author_facet | Li, Changyou Li, Siyuan Jia, Changkai Yang, Lingling Song, Zicheng Wang, Yiqiang |
author_sort | Li, Changyou |
collection | PubMed |
description | Previous studies showed that several members of the S100A family are involved in neovascularization and tumor development. This study checked whether low concentrations of S100A8 or S100A9 has any effect on the behaviour of vascular endothelial cells. A human umbilical vascular endothelial cell (HUVEC) line was used to measure vascular endothelial cell bioactivity related to angiogenesis, such as cell proliferation, migration, and vessel formation. In the low concentration range up to 10 μg/mL, either each alone or in combination, S100A8 and S100A9 proteins promoted proliferation of HUVEC cells in a dose-dependent manner. The presence of both proteins in culture showed additive effects over each single protein. Both proteins enhanced HUVEC cells to migrate across the transwell membrane and to form tube-like structures on the Matrigel surface. When mixed in Matrigel and injected subcutaneously in Balb/c mice, both proteins increased vessel development in the gel plugs. Microarray assay of HUVEC cells treated with 10 μg/mL S100A8 revealed that ribosome pathway, pathogenic Escherichia coli infection pathway, apoptosis, and stress response genes were modulated by S100A8 treatment. We propose that S100A8 and S100A9 proteins from either infiltrating inflammatory cells or tumor cells play an important role in the interplay among inflammation, angiogenesis, and tumorigenesis. |
format | Online Article Text |
id | pubmed-3363068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33630682012-06-08 Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? Li, Changyou Li, Siyuan Jia, Changkai Yang, Lingling Song, Zicheng Wang, Yiqiang Mediators Inflamm Research Article Previous studies showed that several members of the S100A family are involved in neovascularization and tumor development. This study checked whether low concentrations of S100A8 or S100A9 has any effect on the behaviour of vascular endothelial cells. A human umbilical vascular endothelial cell (HUVEC) line was used to measure vascular endothelial cell bioactivity related to angiogenesis, such as cell proliferation, migration, and vessel formation. In the low concentration range up to 10 μg/mL, either each alone or in combination, S100A8 and S100A9 proteins promoted proliferation of HUVEC cells in a dose-dependent manner. The presence of both proteins in culture showed additive effects over each single protein. Both proteins enhanced HUVEC cells to migrate across the transwell membrane and to form tube-like structures on the Matrigel surface. When mixed in Matrigel and injected subcutaneously in Balb/c mice, both proteins increased vessel development in the gel plugs. Microarray assay of HUVEC cells treated with 10 μg/mL S100A8 revealed that ribosome pathway, pathogenic Escherichia coli infection pathway, apoptosis, and stress response genes were modulated by S100A8 treatment. We propose that S100A8 and S100A9 proteins from either infiltrating inflammatory cells or tumor cells play an important role in the interplay among inflammation, angiogenesis, and tumorigenesis. Hindawi Publishing Corporation 2012 2012-05-17 /pmc/articles/PMC3363068/ /pubmed/22685372 http://dx.doi.org/10.1155/2012/248574 Text en Copyright © 2012 Changyou Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Changyou Li, Siyuan Jia, Changkai Yang, Lingling Song, Zicheng Wang, Yiqiang Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title | Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title_full | Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title_fullStr | Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title_full_unstemmed | Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title_short | Low Concentration of S100A8/9 Promotes Angiogenesis-Related Activity of Vascular Endothelial Cells: Bridges among Inflammation, Angiogenesis, and Tumorigenesis? |
title_sort | low concentration of s100a8/9 promotes angiogenesis-related activity of vascular endothelial cells: bridges among inflammation, angiogenesis, and tumorigenesis? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3363068/ https://www.ncbi.nlm.nih.gov/pubmed/22685372 http://dx.doi.org/10.1155/2012/248574 |
work_keys_str_mv | AT lichangyou lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis AT lisiyuan lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis AT jiachangkai lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis AT yanglingling lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis AT songzicheng lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis AT wangyiqiang lowconcentrationofs100a89promotesangiogenesisrelatedactivityofvascularendothelialcellsbridgesamonginflammationangiogenesisandtumorigenesis |