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The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria

Crista junctions (CJs) are tubular invaginations of the inner membrane of mitochondria that connect the inner boundary with the cristae membrane. These architectural elements are critical for mitochondrial function. The yeast inner membrane protein Fcj1, called mitofilin in mammals, was reported to...

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Autores principales: Körner, Christian, Barrera, Miguel, Dukanovic, Jovana, Eydt, Katharina, Harner, Max, Rabl, Regina, Vogel, Frank, Rapaport, Doron, Neupert, Walter, Reichert, Andreas S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364178/
https://www.ncbi.nlm.nih.gov/pubmed/22496419
http://dx.doi.org/10.1091/mbc.E11-10-0831
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author Körner, Christian
Barrera, Miguel
Dukanovic, Jovana
Eydt, Katharina
Harner, Max
Rabl, Regina
Vogel, Frank
Rapaport, Doron
Neupert, Walter
Reichert, Andreas S.
author_facet Körner, Christian
Barrera, Miguel
Dukanovic, Jovana
Eydt, Katharina
Harner, Max
Rabl, Regina
Vogel, Frank
Rapaport, Doron
Neupert, Walter
Reichert, Andreas S.
author_sort Körner, Christian
collection PubMed
description Crista junctions (CJs) are tubular invaginations of the inner membrane of mitochondria that connect the inner boundary with the cristae membrane. These architectural elements are critical for mitochondrial function. The yeast inner membrane protein Fcj1, called mitofilin in mammals, was reported to be preferentially located at CJs and crucial for their formation. Here we investigate the functional roles of individual domains of Fcj1. The most conserved part of Fcj1, the C-terminal domain, is essential for Fcj1 function. In its absence, formation of CJ is strongly impaired and irregular, and stacked cristae are present. This domain interacts with full-length Fcj1, suggesting a role in oligomer formation. It also interacts with Tob55 of the translocase of outer membrane β-barrel proteins (TOB)/sorting and assembly machinery (SAM) complex, which is required for the insertion of β-barrel proteins into the outer membrane. The association of the TOB/SAM complex with contact sites depends on the presence of Fcj1. The biogenesis of β-barrel proteins is not significantly affected in the absence of Fcj1. However, down-regulation of the TOB/SAM complex leads to altered cristae morphology and a moderate reduction in the number of CJs. We propose that the C-terminal domain of Fcj1 is critical for the interaction of Fcj1 with the TOB/SAM complex and thereby for stabilizing CJs in close proximity to the outer membrane. These results assign novel functions to both the C-terminal domain of Fcj1 and the TOB/SAM complex.
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spelling pubmed-33641782012-08-16 The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria Körner, Christian Barrera, Miguel Dukanovic, Jovana Eydt, Katharina Harner, Max Rabl, Regina Vogel, Frank Rapaport, Doron Neupert, Walter Reichert, Andreas S. Mol Biol Cell Articles Crista junctions (CJs) are tubular invaginations of the inner membrane of mitochondria that connect the inner boundary with the cristae membrane. These architectural elements are critical for mitochondrial function. The yeast inner membrane protein Fcj1, called mitofilin in mammals, was reported to be preferentially located at CJs and crucial for their formation. Here we investigate the functional roles of individual domains of Fcj1. The most conserved part of Fcj1, the C-terminal domain, is essential for Fcj1 function. In its absence, formation of CJ is strongly impaired and irregular, and stacked cristae are present. This domain interacts with full-length Fcj1, suggesting a role in oligomer formation. It also interacts with Tob55 of the translocase of outer membrane β-barrel proteins (TOB)/sorting and assembly machinery (SAM) complex, which is required for the insertion of β-barrel proteins into the outer membrane. The association of the TOB/SAM complex with contact sites depends on the presence of Fcj1. The biogenesis of β-barrel proteins is not significantly affected in the absence of Fcj1. However, down-regulation of the TOB/SAM complex leads to altered cristae morphology and a moderate reduction in the number of CJs. We propose that the C-terminal domain of Fcj1 is critical for the interaction of Fcj1 with the TOB/SAM complex and thereby for stabilizing CJs in close proximity to the outer membrane. These results assign novel functions to both the C-terminal domain of Fcj1 and the TOB/SAM complex. The American Society for Cell Biology 2012-06-01 /pmc/articles/PMC3364178/ /pubmed/22496419 http://dx.doi.org/10.1091/mbc.E11-10-0831 Text en © 2012 Körner et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Körner, Christian
Barrera, Miguel
Dukanovic, Jovana
Eydt, Katharina
Harner, Max
Rabl, Regina
Vogel, Frank
Rapaport, Doron
Neupert, Walter
Reichert, Andreas S.
The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title_full The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title_fullStr The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title_full_unstemmed The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title_short The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria
title_sort c-terminal domain of fcj1 is required for formation of crista junctions and interacts with the tob/sam complex in mitochondria
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364178/
https://www.ncbi.nlm.nih.gov/pubmed/22496419
http://dx.doi.org/10.1091/mbc.E11-10-0831
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