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Fluorescence Polarization and Fluctuation Analysis Monitors Subunit Proximity, Stoichiometry, and Protein Complex Hydrodynamics

Förster resonance energy transfer (FRET) microscopy is frequently used to study protein interactions and conformational changes in living cells. The utility of FRET is limited by false positive and negative signals. To overcome these limitations we have developed Fluorescence Polarization and Fluctu...

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Detalles Bibliográficos
Autores principales:	Nguyen, Tuan A., Sarkar, Pabak, Veetil, Jithesh V., Koushik, Srinagesh V., Vogel, Steven S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364239/ https://www.ncbi.nlm.nih.gov/pubmed/22666486 http://dx.doi.org/10.1371/journal.pone.0038209

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