Cargando…
14-3-3ε Is Required for Germ Cell Migration in Drosophila
Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redunda...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364263/ https://www.ncbi.nlm.nih.gov/pubmed/22666326 http://dx.doi.org/10.1371/journal.pone.0036702 |
_version_ | 1782234517803630592 |
---|---|
author | Tsigkari, K. Kirki Acevedo, Summer F. Skoulakis, Efthimios M. C. |
author_facet | Tsigkari, K. Kirki Acevedo, Summer F. Skoulakis, Efthimios M. C. |
author_sort | Tsigkari, K. Kirki |
collection | PubMed |
description | Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells. |
format | Online Article Text |
id | pubmed-3364263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33642632012-06-04 14-3-3ε Is Required for Germ Cell Migration in Drosophila Tsigkari, K. Kirki Acevedo, Summer F. Skoulakis, Efthimios M. C. PLoS One Research Article Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells. Public Library of Science 2012-05-30 /pmc/articles/PMC3364263/ /pubmed/22666326 http://dx.doi.org/10.1371/journal.pone.0036702 Text en Tsigkari et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tsigkari, K. Kirki Acevedo, Summer F. Skoulakis, Efthimios M. C. 14-3-3ε Is Required for Germ Cell Migration in Drosophila |
title | 14-3-3ε Is Required for Germ Cell Migration in Drosophila
|
title_full | 14-3-3ε Is Required for Germ Cell Migration in Drosophila
|
title_fullStr | 14-3-3ε Is Required for Germ Cell Migration in Drosophila
|
title_full_unstemmed | 14-3-3ε Is Required for Germ Cell Migration in Drosophila
|
title_short | 14-3-3ε Is Required for Germ Cell Migration in Drosophila
|
title_sort | 14-3-3ε is required for germ cell migration in drosophila |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364263/ https://www.ncbi.nlm.nih.gov/pubmed/22666326 http://dx.doi.org/10.1371/journal.pone.0036702 |
work_keys_str_mv | AT tsigkarikkirki 1433eisrequiredforgermcellmigrationindrosophila AT acevedosummerf 1433eisrequiredforgermcellmigrationindrosophila AT skoulakisefthimiosmc 1433eisrequiredforgermcellmigrationindrosophila |