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14-3-3ε Is Required for Germ Cell Migration in Drosophila

Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redunda...

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Autores principales: Tsigkari, K. Kirki, Acevedo, Summer F., Skoulakis, Efthimios M. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364263/
https://www.ncbi.nlm.nih.gov/pubmed/22666326
http://dx.doi.org/10.1371/journal.pone.0036702
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author Tsigkari, K. Kirki
Acevedo, Summer F.
Skoulakis, Efthimios M. C.
author_facet Tsigkari, K. Kirki
Acevedo, Summer F.
Skoulakis, Efthimios M. C.
author_sort Tsigkari, K. Kirki
collection PubMed
description Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells.
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spelling pubmed-33642632012-06-04 14-3-3ε Is Required for Germ Cell Migration in Drosophila Tsigkari, K. Kirki Acevedo, Summer F. Skoulakis, Efthimios M. C. PLoS One Research Article Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells. Public Library of Science 2012-05-30 /pmc/articles/PMC3364263/ /pubmed/22666326 http://dx.doi.org/10.1371/journal.pone.0036702 Text en Tsigkari et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tsigkari, K. Kirki
Acevedo, Summer F.
Skoulakis, Efthimios M. C.
14-3-3ε Is Required for Germ Cell Migration in Drosophila
title 14-3-3ε Is Required for Germ Cell Migration in Drosophila
title_full 14-3-3ε Is Required for Germ Cell Migration in Drosophila
title_fullStr 14-3-3ε Is Required for Germ Cell Migration in Drosophila
title_full_unstemmed 14-3-3ε Is Required for Germ Cell Migration in Drosophila
title_short 14-3-3ε Is Required for Germ Cell Migration in Drosophila
title_sort 14-3-3ε is required for germ cell migration in drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364263/
https://www.ncbi.nlm.nih.gov/pubmed/22666326
http://dx.doi.org/10.1371/journal.pone.0036702
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