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Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation
Crop production on soils containing sub-optimal levels of nitrogen (N) severely compromises yield potential. The development of plant varieties displaying high N use efficiency (NUE) will optimize N fertilizer use and reduce the environmental damage caused by excess N application. Maize is one of th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364463/ https://www.ncbi.nlm.nih.gov/pubmed/22666225 http://dx.doi.org/10.3389/fpls.2011.00049 |
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author | El-kereamy, Ashraf Guevara, David Bi, Yong-Mei Chen, Xi Rothstein, Steven J. |
author_facet | El-kereamy, Ashraf Guevara, David Bi, Yong-Mei Chen, Xi Rothstein, Steven J. |
author_sort | El-kereamy, Ashraf |
collection | PubMed |
description | Crop production on soils containing sub-optimal levels of nitrogen (N) severely compromises yield potential. The development of plant varieties displaying high N use efficiency (NUE) will optimize N fertilizer use and reduce the environmental damage caused by excess N application. Maize is one of the most important crops cultivated worldwide. Identification of the genotypes with an enhanced NUE in the field is both time and resource consuming and sometime is difficult due to the regulation in the biotechnology programs. Identification of traits associated with adaptation to N limitation at an early vegetative stage which could reflect NUE at maturity is in need. We developed a hydroponic growth system and used it to test two genotypes that were different in their NUE at maturity under N limitation. One genotype SRG-200 showed a higher NUE than the other genotype SRG-100 and we used its hybrid SRG-150 as a reference for NUE. A number of phenotypic, molecular, and metabolic factors were tested using these three genetic lines at an early vegetative stage to determine which of these could be more indicative of predicting improved NUE at an early seedling stage. These include a transcriptional analysis which showed that the higher NUE in SRG-200 genotype is associated with higher transcript levels for the genes involved in nitrate transport, N assimilation, and GS and that the SRG-200 genotype maintained higher sugar content in leaves. Those identified in this study could be useful indicators for selecting promising maize lines at early stages to help develop elite varieties showing an enhanced NUE. |
format | Online Article Text |
id | pubmed-3364463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33644632012-06-04 Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation El-kereamy, Ashraf Guevara, David Bi, Yong-Mei Chen, Xi Rothstein, Steven J. Front Plant Sci Plant Science Crop production on soils containing sub-optimal levels of nitrogen (N) severely compromises yield potential. The development of plant varieties displaying high N use efficiency (NUE) will optimize N fertilizer use and reduce the environmental damage caused by excess N application. Maize is one of the most important crops cultivated worldwide. Identification of the genotypes with an enhanced NUE in the field is both time and resource consuming and sometime is difficult due to the regulation in the biotechnology programs. Identification of traits associated with adaptation to N limitation at an early vegetative stage which could reflect NUE at maturity is in need. We developed a hydroponic growth system and used it to test two genotypes that were different in their NUE at maturity under N limitation. One genotype SRG-200 showed a higher NUE than the other genotype SRG-100 and we used its hybrid SRG-150 as a reference for NUE. A number of phenotypic, molecular, and metabolic factors were tested using these three genetic lines at an early vegetative stage to determine which of these could be more indicative of predicting improved NUE at an early seedling stage. These include a transcriptional analysis which showed that the higher NUE in SRG-200 genotype is associated with higher transcript levels for the genes involved in nitrate transport, N assimilation, and GS and that the SRG-200 genotype maintained higher sugar content in leaves. Those identified in this study could be useful indicators for selecting promising maize lines at early stages to help develop elite varieties showing an enhanced NUE. Frontiers Research Foundation 2011-09-13 /pmc/articles/PMC3364463/ /pubmed/22666225 http://dx.doi.org/10.3389/fpls.2011.00049 Text en Copyright © 2011 El-kereamy, Guevara, Bi, Chen and Rothstein. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with. |
spellingShingle | Plant Science El-kereamy, Ashraf Guevara, David Bi, Yong-Mei Chen, Xi Rothstein, Steven J. Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title | Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title_full | Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title_fullStr | Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title_full_unstemmed | Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title_short | Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation |
title_sort | exploring the molecular and metabolic factors contributing to the adaptation of maize seedlings to nitrate limitation |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364463/ https://www.ncbi.nlm.nih.gov/pubmed/22666225 http://dx.doi.org/10.3389/fpls.2011.00049 |
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