Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections

Scanning small and wide angle X-ray scattering (scanning SWAXS) experiments were performed on healthy and pathologic human bone sections. Via crystallographic tools the data were transformed into quantitative images and as such compared with circularly polarized light (CPL) microscopy images. SWAXS...

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Autores principales: Giannini, C., Siliqi, D., Bunk, O., Beraudi, A., Ladisa, M., Altamura, D., Stea, S., Baruffaldi, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364490/
https://www.ncbi.nlm.nih.gov/pubmed/22666538
http://dx.doi.org/10.1038/srep00435
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author Giannini, C.
Siliqi, D.
Bunk, O.
Beraudi, A.
Ladisa, M.
Altamura, D.
Stea, S.
Baruffaldi, F.
author_facet Giannini, C.
Siliqi, D.
Bunk, O.
Beraudi, A.
Ladisa, M.
Altamura, D.
Stea, S.
Baruffaldi, F.
author_sort Giannini, C.
collection PubMed
description Scanning small and wide angle X-ray scattering (scanning SWAXS) experiments were performed on healthy and pathologic human bone sections. Via crystallographic tools the data were transformed into quantitative images and as such compared with circularly polarized light (CPL) microscopy images. SWAXS and CPL images allowed extracting information of the mineral nanocrystalline phase embedded, with and without preferred orientation, in the collagen fibrils, mapping local changes at sub-osteon resolution. This favorable combination has been applied for the first time to biopsies of dwarfism syndrome and Paget's disease to shed light onto the cortical structure of natural bone in healthy and pathologic sections.
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spelling pubmed-33644902012-06-04 Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections Giannini, C. Siliqi, D. Bunk, O. Beraudi, A. Ladisa, M. Altamura, D. Stea, S. Baruffaldi, F. Sci Rep Article Scanning small and wide angle X-ray scattering (scanning SWAXS) experiments were performed on healthy and pathologic human bone sections. Via crystallographic tools the data were transformed into quantitative images and as such compared with circularly polarized light (CPL) microscopy images. SWAXS and CPL images allowed extracting information of the mineral nanocrystalline phase embedded, with and without preferred orientation, in the collagen fibrils, mapping local changes at sub-osteon resolution. This favorable combination has been applied for the first time to biopsies of dwarfism syndrome and Paget's disease to shed light onto the cortical structure of natural bone in healthy and pathologic sections. Nature Publishing Group 2012-05-31 /pmc/articles/PMC3364490/ /pubmed/22666538 http://dx.doi.org/10.1038/srep00435 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Giannini, C.
Siliqi, D.
Bunk, O.
Beraudi, A.
Ladisa, M.
Altamura, D.
Stea, S.
Baruffaldi, F.
Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title_full Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title_fullStr Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title_full_unstemmed Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title_short Correlative Light and Scanning X-Ray Scattering Microscopy of Healthy and Pathologic Human Bone Sections
title_sort correlative light and scanning x-ray scattering microscopy of healthy and pathologic human bone sections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364490/
https://www.ncbi.nlm.nih.gov/pubmed/22666538
http://dx.doi.org/10.1038/srep00435
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