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Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat

BACKGROUND: The usefulness of the typical direct methods involving duodenal intubation, such as the secretin and secretin-cholecystokinin tests, in the diagnosis of exocrine pancreatic dysfunction is widely accepted. However, these diagnostic tests tend to be avoided because of their technical compl...

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Autores principales: Urita, Yoshihisa, Watanabe, Toshiyasu, Imai, Tsunehiko, Miura, Yasuyuki, Honda, Yoshiko, Washizawa, Naohiro, Sanaka, Masaki, Shimada, Nagato, Nakajima, Hitoshi, Sugimoto, Motonobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364616/
https://www.ncbi.nlm.nih.gov/pubmed/22666702
http://dx.doi.org/10.4297/najms.2009.5239
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author Urita, Yoshihisa
Watanabe, Toshiyasu
Imai, Tsunehiko
Miura, Yasuyuki
Honda, Yoshiko
Washizawa, Naohiro
Sanaka, Masaki
Shimada, Nagato
Nakajima, Hitoshi
Sugimoto, Motonobu
author_facet Urita, Yoshihisa
Watanabe, Toshiyasu
Imai, Tsunehiko
Miura, Yasuyuki
Honda, Yoshiko
Washizawa, Naohiro
Sanaka, Masaki
Shimada, Nagato
Nakajima, Hitoshi
Sugimoto, Motonobu
author_sort Urita, Yoshihisa
collection PubMed
description BACKGROUND: The usefulness of the typical direct methods involving duodenal intubation, such as the secretin and secretin-cholecystokinin tests, in the diagnosis of exocrine pancreatic dysfunction is widely accepted. However, these diagnostic tests tend to be avoided because of their technical complexity and the burden on patients. Recently, a simple breath test was developed for assessment of exocrine pancreatic function employing 13C-dipeptide [i.e., benzoyl-L-tyrosyl-[1-13C] alanine (Bz-Tyr-Ala)]. Although alcohol abuse causes pancreatic damage in humans, this has been unclear in rats. AIMS: The aim of the study is to evaluate the effect of ethanol exposure beginning at an early age on extra-pancreatic secretory function in rats. MATERIALS AND METHODS: Twelve female rats of the F344 strain aged 12 months were used. Seven rats were fed on a commercial mash food with 16% ethanol solution (Japanese Sake) as drinking-fluid since at 29 days of age (ethanol group). The remaining five rats were fed on a nutrient-matched isocaloric diet with water as drinking-fluid (control group). After 24-hr fasting, rats are orally administrated 1cc of water containing sodium 13C-dipeptide (5 mg/kg) and housed in an animal chamber. The expired air in the chamber is collected in a breath-sampling bag using a tube and aspiration pump. The 13CO2 concentration is measured using an infrared spectrometer at 10-min interval for 120 min and expressed as delta per mil. RESULTS: The breath 13CO2 level increased and peaked at 20 min in both two groups. In general, 13CO2 excretion peaked rapidly and also decreased sooner in ethanol rats than in control rats. The mean value of the maximal 13CO2 excretion is 34.7 per mil in ethanol rats, greater than in control rats (31.4 per mil), but the difference did not reach the statistically significance. CONCLUSION: Chronic ethanol feeding beginning at an early age does not affect extra-pancreatic secretory function in rats.
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spelling pubmed-33646162012-06-04 Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat Urita, Yoshihisa Watanabe, Toshiyasu Imai, Tsunehiko Miura, Yasuyuki Honda, Yoshiko Washizawa, Naohiro Sanaka, Masaki Shimada, Nagato Nakajima, Hitoshi Sugimoto, Motonobu N Am J Med Sci Original Article BACKGROUND: The usefulness of the typical direct methods involving duodenal intubation, such as the secretin and secretin-cholecystokinin tests, in the diagnosis of exocrine pancreatic dysfunction is widely accepted. However, these diagnostic tests tend to be avoided because of their technical complexity and the burden on patients. Recently, a simple breath test was developed for assessment of exocrine pancreatic function employing 13C-dipeptide [i.e., benzoyl-L-tyrosyl-[1-13C] alanine (Bz-Tyr-Ala)]. Although alcohol abuse causes pancreatic damage in humans, this has been unclear in rats. AIMS: The aim of the study is to evaluate the effect of ethanol exposure beginning at an early age on extra-pancreatic secretory function in rats. MATERIALS AND METHODS: Twelve female rats of the F344 strain aged 12 months were used. Seven rats were fed on a commercial mash food with 16% ethanol solution (Japanese Sake) as drinking-fluid since at 29 days of age (ethanol group). The remaining five rats were fed on a nutrient-matched isocaloric diet with water as drinking-fluid (control group). After 24-hr fasting, rats are orally administrated 1cc of water containing sodium 13C-dipeptide (5 mg/kg) and housed in an animal chamber. The expired air in the chamber is collected in a breath-sampling bag using a tube and aspiration pump. The 13CO2 concentration is measured using an infrared spectrometer at 10-min interval for 120 min and expressed as delta per mil. RESULTS: The breath 13CO2 level increased and peaked at 20 min in both two groups. In general, 13CO2 excretion peaked rapidly and also decreased sooner in ethanol rats than in control rats. The mean value of the maximal 13CO2 excretion is 34.7 per mil in ethanol rats, greater than in control rats (31.4 per mil), but the difference did not reach the statistically significance. CONCLUSION: Chronic ethanol feeding beginning at an early age does not affect extra-pancreatic secretory function in rats. Medknow Publications & Media Pvt Ltd 2009-10 /pmc/articles/PMC3364616/ /pubmed/22666702 http://dx.doi.org/10.4297/najms.2009.5239 Text en Copyright: © North American Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Urita, Yoshihisa
Watanabe, Toshiyasu
Imai, Tsunehiko
Miura, Yasuyuki
Honda, Yoshiko
Washizawa, Naohiro
Sanaka, Masaki
Shimada, Nagato
Nakajima, Hitoshi
Sugimoto, Motonobu
Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title_full Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title_fullStr Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title_full_unstemmed Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title_short Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
title_sort influence of chronic ethanol consumption on extra-pancreatic secretory function in rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364616/
https://www.ncbi.nlm.nih.gov/pubmed/22666702
http://dx.doi.org/10.4297/najms.2009.5239
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