Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction

BACKGROUND: Psychological stress is one of the factors associated with many human diseases; the mechanisms need to be further understood. METHODS: Rats were subjected to chronic water avoid stress. Intestinal epithelial heat shock protein (HSP) 70 was evaluated. The intestinal epithelial permeabilit...

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Autores principales: Yang, Ping-Chang, Tu, Ya-Hong, Perdue, Mary H., Oluwole, Christine, Struiksma, Stevie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364627/
https://www.ncbi.nlm.nih.gov/pubmed/22666665
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author Yang, Ping-Chang
Tu, Ya-Hong
Perdue, Mary H.
Oluwole, Christine
Struiksma, Stevie
author_facet Yang, Ping-Chang
Tu, Ya-Hong
Perdue, Mary H.
Oluwole, Christine
Struiksma, Stevie
author_sort Yang, Ping-Chang
collection PubMed
description BACKGROUND: Psychological stress is one of the factors associated with many human diseases; the mechanisms need to be further understood. METHODS: Rats were subjected to chronic water avoid stress. Intestinal epithelial heat shock protein (HSP) 70 was evaluated. The intestinal epithelial permeability was examined with Ussing chamber technique. RESULTS: HSP70 was detected in normal intestinal epithelial cells. Psychological stress decreased HSP70 in the intestinal epithelial cells that correlated with the stress-induced intestinal epithelial hyperpermeability. Pretreatment with HSP70 abrogated stress-induced intestinal barrier dysfunction. CONCLUSIONS: Chronic stress inhibits HSP70 activity in rat intestinal epithelial layer that is associated with intestinal epithelial barrier dysfunction, which can be prevented by pretreatment with HSP70 protein. (Yang PC, Tu YH, Perdue MH, Oluwole C, Struiksma S. Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction.
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spelling pubmed-33646272012-06-04 Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction Yang, Ping-Chang Tu, Ya-Hong Perdue, Mary H. Oluwole, Christine Struiksma, Stevie N Am J Med Sci Research Article BACKGROUND: Psychological stress is one of the factors associated with many human diseases; the mechanisms need to be further understood. METHODS: Rats were subjected to chronic water avoid stress. Intestinal epithelial heat shock protein (HSP) 70 was evaluated. The intestinal epithelial permeability was examined with Ussing chamber technique. RESULTS: HSP70 was detected in normal intestinal epithelial cells. Psychological stress decreased HSP70 in the intestinal epithelial cells that correlated with the stress-induced intestinal epithelial hyperpermeability. Pretreatment with HSP70 abrogated stress-induced intestinal barrier dysfunction. CONCLUSIONS: Chronic stress inhibits HSP70 activity in rat intestinal epithelial layer that is associated with intestinal epithelial barrier dysfunction, which can be prevented by pretreatment with HSP70 protein. (Yang PC, Tu YH, Perdue MH, Oluwole C, Struiksma S. Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction. Medknow Publications & Media Pvt Ltd 2009-06 /pmc/articles/PMC3364627/ /pubmed/22666665 Text en Copyright: © North American Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Ping-Chang
Tu, Ya-Hong
Perdue, Mary H.
Oluwole, Christine
Struiksma, Stevie
Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title_full Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title_fullStr Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title_full_unstemmed Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title_short Regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
title_sort regulatory effect of heat shock protein 70 in stress-induced rat intestinal epithelial barrier dysfunction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364627/
https://www.ncbi.nlm.nih.gov/pubmed/22666665
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