High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants

With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for making hairpin RNA (hpRNA) constructs is in great demand. Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction o...

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Detalles Bibliográficos
Autores principales: Yan, Pu, Shen, Wentao, Gao, XinZheng, Li, Xiaoying, Zhou, Peng, Duan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364983/
https://www.ncbi.nlm.nih.gov/pubmed/22675447
http://dx.doi.org/10.1371/journal.pone.0038186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364983/
https://www.ncbi.nlm.nih.gov/pubmed/22675447
http://dx.doi.org/10.1371/journal.pone.0038186

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