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High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants
With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for making hairpin RNA (hpRNA) constructs is in great demand. Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction o...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364983/ https://www.ncbi.nlm.nih.gov/pubmed/22675447 http://dx.doi.org/10.1371/journal.pone.0038186 |