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Generation of Induced Pluripotent Stem Cells from the Prairie Vole
The vast majority of animals mate more or less promiscuously. A few mammals, including humans, utilize more restrained mating strategies that entail a longer term affiliation with a single mating partner. Such pair bonding mating strategies have been resistant to genetic analysis because of a lack o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365000/ https://www.ncbi.nlm.nih.gov/pubmed/22675440 http://dx.doi.org/10.1371/journal.pone.0038119 |
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author | Manoli, Devanand S. Subramanyam, Deepa Carey, Catriona Sudin, Erik Van Westerhuyzen, Julie A. Bales, Karen L. Blelloch, Robert Shah, Nirao M. |
author_facet | Manoli, Devanand S. Subramanyam, Deepa Carey, Catriona Sudin, Erik Van Westerhuyzen, Julie A. Bales, Karen L. Blelloch, Robert Shah, Nirao M. |
author_sort | Manoli, Devanand S. |
collection | PubMed |
description | The vast majority of animals mate more or less promiscuously. A few mammals, including humans, utilize more restrained mating strategies that entail a longer term affiliation with a single mating partner. Such pair bonding mating strategies have been resistant to genetic analysis because of a lack of suitable model organisms. Prairie voles are small mouse-like rodents that form enduring pair bonds in the wild as well as in the laboratory, and consequently they have been used widely to study social bonding behavior. The lack of targeted genetic approaches in this species however has restricted the study of the molecular and neural circuit basis of pair bonds. As a first step in rendering the prairie vole amenable to reverse genetics, we have generated induced pluripotent stem cell (IPSC) lines from prairie vole fibroblasts using retroviral transduction of reprogramming factors. These IPSC lines display the cellular and molecular hallmarks of IPSC cells from other organisms, including mice and humans. Moreover, the prairie vole IPSC lines have pluripotent differentiation potential since they can give rise to all three germ layers in tissue culture and in vivo. These IPSC lines can now be used to develop conditions that facilitate homologous recombination and eventually the generation of prairie voles bearing targeted genetic modifications to study the molecular and neural basis of pair bond formation. |
format | Online Article Text |
id | pubmed-3365000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33650002012-06-06 Generation of Induced Pluripotent Stem Cells from the Prairie Vole Manoli, Devanand S. Subramanyam, Deepa Carey, Catriona Sudin, Erik Van Westerhuyzen, Julie A. Bales, Karen L. Blelloch, Robert Shah, Nirao M. PLoS One Research Article The vast majority of animals mate more or less promiscuously. A few mammals, including humans, utilize more restrained mating strategies that entail a longer term affiliation with a single mating partner. Such pair bonding mating strategies have been resistant to genetic analysis because of a lack of suitable model organisms. Prairie voles are small mouse-like rodents that form enduring pair bonds in the wild as well as in the laboratory, and consequently they have been used widely to study social bonding behavior. The lack of targeted genetic approaches in this species however has restricted the study of the molecular and neural circuit basis of pair bonds. As a first step in rendering the prairie vole amenable to reverse genetics, we have generated induced pluripotent stem cell (IPSC) lines from prairie vole fibroblasts using retroviral transduction of reprogramming factors. These IPSC lines display the cellular and molecular hallmarks of IPSC cells from other organisms, including mice and humans. Moreover, the prairie vole IPSC lines have pluripotent differentiation potential since they can give rise to all three germ layers in tissue culture and in vivo. These IPSC lines can now be used to develop conditions that facilitate homologous recombination and eventually the generation of prairie voles bearing targeted genetic modifications to study the molecular and neural basis of pair bond formation. Public Library of Science 2012-05-31 /pmc/articles/PMC3365000/ /pubmed/22675440 http://dx.doi.org/10.1371/journal.pone.0038119 Text en Manoli et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Manoli, Devanand S. Subramanyam, Deepa Carey, Catriona Sudin, Erik Van Westerhuyzen, Julie A. Bales, Karen L. Blelloch, Robert Shah, Nirao M. Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title | Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title_full | Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title_fullStr | Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title_full_unstemmed | Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title_short | Generation of Induced Pluripotent Stem Cells from the Prairie Vole |
title_sort | generation of induced pluripotent stem cells from the prairie vole |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365000/ https://www.ncbi.nlm.nih.gov/pubmed/22675440 http://dx.doi.org/10.1371/journal.pone.0038119 |
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