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Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establi...
Autores principales: | Haruki, Hirohito, Gonzalez, Monica Rengifo, Johnsson, Kai |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365113/ https://www.ncbi.nlm.nih.gov/pubmed/22701522 http://dx.doi.org/10.1371/journal.pone.0037598 |
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