Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure

The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeod...

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Autores principales: Domingues, Nuno, Matos, Ana Rita, Marques da Silva, Jorge, Cartaxana, Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365893/
https://www.ncbi.nlm.nih.gov/pubmed/22675519
http://dx.doi.org/10.1371/journal.pone.0038162
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author Domingues, Nuno
Matos, Ana Rita
Marques da Silva, Jorge
Cartaxana, Paulo
author_facet Domingues, Nuno
Matos, Ana Rita
Marques da Silva, Jorge
Cartaxana, Paulo
author_sort Domingues, Nuno
collection PubMed
description The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m(−2) s(−1)) subjected to high light (HL, 1,250 µmol photons m(−2) s(−1)) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (F (v)/F (m)), showing a reduction of active PSII reaction centres. Partial recovery of F (v)/F (m) in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition.
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spelling pubmed-33658932012-06-06 Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure Domingues, Nuno Matos, Ana Rita Marques da Silva, Jorge Cartaxana, Paulo PLoS One Research Article The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m(−2) s(−1)) subjected to high light (HL, 1,250 µmol photons m(−2) s(−1)) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (F (v)/F (m)), showing a reduction of active PSII reaction centres. Partial recovery of F (v)/F (m) in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition. Public Library of Science 2012-06-01 /pmc/articles/PMC3365893/ /pubmed/22675519 http://dx.doi.org/10.1371/journal.pone.0038162 Text en Domingues et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Domingues, Nuno
Matos, Ana Rita
Marques da Silva, Jorge
Cartaxana, Paulo
Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title_full Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title_fullStr Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title_full_unstemmed Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title_short Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
title_sort response of the diatom phaeodactylum tricornutum to photooxidative stress resulting from high light exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365893/
https://www.ncbi.nlm.nih.gov/pubmed/22675519
http://dx.doi.org/10.1371/journal.pone.0038162
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