Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeod...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365893/ https://www.ncbi.nlm.nih.gov/pubmed/22675519 http://dx.doi.org/10.1371/journal.pone.0038162 |
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author | Domingues, Nuno Matos, Ana Rita Marques da Silva, Jorge Cartaxana, Paulo |
author_facet | Domingues, Nuno Matos, Ana Rita Marques da Silva, Jorge Cartaxana, Paulo |
author_sort | Domingues, Nuno |
collection | PubMed |
description | The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m(−2) s(−1)) subjected to high light (HL, 1,250 µmol photons m(−2) s(−1)) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (F (v)/F (m)), showing a reduction of active PSII reaction centres. Partial recovery of F (v)/F (m) in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition. |
format | Online Article Text |
id | pubmed-3365893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33658932012-06-06 Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure Domingues, Nuno Matos, Ana Rita Marques da Silva, Jorge Cartaxana, Paulo PLoS One Research Article The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m(−2) s(−1)) subjected to high light (HL, 1,250 µmol photons m(−2) s(−1)) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (F (v)/F (m)), showing a reduction of active PSII reaction centres. Partial recovery of F (v)/F (m) in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition. Public Library of Science 2012-06-01 /pmc/articles/PMC3365893/ /pubmed/22675519 http://dx.doi.org/10.1371/journal.pone.0038162 Text en Domingues et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Domingues, Nuno Matos, Ana Rita Marques da Silva, Jorge Cartaxana, Paulo Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title | Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title_full | Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title_fullStr | Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title_full_unstemmed | Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title_short | Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure |
title_sort | response of the diatom phaeodactylum tricornutum to photooxidative stress resulting from high light exposure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365893/ https://www.ncbi.nlm.nih.gov/pubmed/22675519 http://dx.doi.org/10.1371/journal.pone.0038162 |
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